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| Status |
Public on Feb 27, 2015 |
| Title |
A Circult Mechanism for Differentiating Positive and Negative Associations |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
The ability to differentiate stimuli predicting positive or negative outcomes is critical for survival, and perturbations of emotional processing underlie many psychiatric disease states. Different neuronal populations of the basolateral amygdala complex (BLA) encode fearful or rewarding associations, but the molecular identity of these functionally distinct populations of BLA neurons remained unknown. Here, we show that BLA neurons projecting to the nucleus accumbens (NAc-projectors) or the centromedial amygdala (CeM-projectors) underwent opposing synaptic changes following fear or reward conditioning. The photostimulation of NAc projectors supported positive reinforcement while photostimulation of CeM projectors mediated negative reinforcement. In search of defining molecular characteristics of these functionally-distinct BLA neuronal populations, we compared gene expression profiles of NAc- and CeM-projectors.
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| Overall design |
For comparison of gene expression profiles of NAc- and CeM-projectors, we conducted two independent RNA sequencing experiments. In experiment-1, a total of n=9 samples (n=4 NAc- and n=5 CeM-projectors) are analyzed. In experiment-2, a total of n=8 samples (n=4 NAc- and n=4 CeM-projectors) are analyzed.
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| Contributor(s) |
Yorozu S, Gray JM |
| Citation(s) |
25925480 |
| Submission date |
Feb 26, 2015 |
| Last update date |
May 15, 2019 |
| Contact name |
Suzuko Yorozu |
| Organization name |
Harvard Medical School
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| Department |
Genetics
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| Lab |
Jesse Gray's lab
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| Street address |
77 Avenue Louis Pasteur
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| City |
Boston |
| State/province |
Massachusettes |
| ZIP/Postal code |
02115 |
| Country |
USA |
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| Platforms (2) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
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| Samples (17)
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| GSM1620043 |
NAc projector, experiment-1: NAc_704 |
| GSM1620044 |
CeM projector, experiment-1: CeM_709 |
| GSM1620045 |
CeM projector, experiment-1: CeM_710 |
| GSM1620046 |
CeM projector, experiment-1: CeM_713 |
| GSM1620047 |
CeM projector, experiment-1: CeM_721 |
| GSM1620048 |
CeM projector, experiment-1: CeM_722 |
| GSM1620049 |
NAc projector, experiment-2: NAc_548 |
| GSM1620050 |
NAc projector, experiment-2: NAc_541 |
| GSM1620051 |
NAc projector, experiment-2: NAc_538 |
| GSM1620052 |
NAc projector, experiment-2: NAc_537 |
| GSM1620053 |
CeM projector, experiment-2: CeM_542 |
| GSM1620054 |
CeM projector, experiment-2: CeM_547 |
| GSM1620055 |
CeM projector, experiment-2: CeM_546 |
| GSM1620056 |
CeM projector, experiment-2: CeM_551 |
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| Relations |
| BioProject |
PRJNA276574 |
| SRA |
SRP055558 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE66345_Summary_HTSeq_output_1stEX.txt.gz |
304.2 Kb |
(ftp)(http) |
TXT |
| GSE66345_Summary_HTSeq_output_2ndEX.txt.gz |
284.7 Kb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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