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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Oct 26, 2014 |
| Title |
Expression data in antigen-specific CD8 T cells in the absence of ATG7 |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Autophagy genes play an important role in the T cell activation and proliferation. We examined the role of ATG7 during the process of CD8 T cell memory formation. In the absence of ATG7, antigen-specific CD8 T cells failed to survive past the contraction phase and failed to give rise to memory cells.
We used microarrays to examine the global programme of gene expression underlying changes introduced in the absence of the ATG7 gene and identified distinct classes genes varied their expression during this time-point.
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| Overall design |
Mouse LCMV-specific CD8 T cells were isolated at day 8 post LCMV infection for RNA extraction and hybridization on Affymetrix microarrays. We sought to compare the gene expression profiles in wild-type cells and cells lacking the ATG7 gene during the peak of their expansion.
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| Contributor(s) |
Xiaojin X, Koichi A, Rafi A |
| Citation(s) |
25362489 |
| Submission date |
Apr 24, 2014 |
| Last update date |
Feb 11, 2019 |
| Contact name |
Xiaojin Xu |
| E-mail(s) |
xiaojinxu@gmail.com
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| Organization name |
Emory University School of Medicine
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| Department |
Emory Vaccine Center and Department of Microbiology and Immunology
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| Lab |
Rafi Ahmed
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| Street address |
1510 Clifton Road
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| City |
Atlanta |
| State/province |
Georgia |
| ZIP/Postal code |
30322 |
| Country |
USA |
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| Platforms (1) |
| GPL1261 |
[Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array |
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| Samples (8)
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| GSM1373715 |
DbGP33+ CD8 T cells, Atg7flox/flox mice, D8, biological rep1 |
| GSM1373716 |
DbGP33+ CD8 T cells, Atg7flox/flox mice, D8, biological rep2 |
| GSM1373717 |
DbGP33+ CD8 T cells, Atg7flox/flox mice, D8, biological rep3 |
| GSM1373718 |
DbGP33+ CD8 T cells, Atg7flox/flox mice, D8, biological rep4 |
| GSM1373719 |
DbGP33+ CD8 T cells, Atg7flox/floxCre mice, Day 8, biological rep1 |
| GSM1373720 |
DbGP33+ CD8 T cells, Atg7flox/floxCre mice, Day 8, biological rep2 |
| GSM1373721 |
DbGP33+ CD8 T cells, Atg7flox/floxCre mice, Day 8, biological rep3 |
| GSM1373722 |
DbGP33+ CD8 T cells, Atg7flox/floxCre mice, Day 8, biological rep4 |
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| Relations |
| BioProject |
PRJNA245319 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE57047_RAW.tar |
32.7 Mb |
(http)(custom) |
TAR (of CEL, CHP) |
| Processed data included within Sample table |
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