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| Status |
Public on Apr 08, 2023 |
| Title |
Spic is required for the differentiation of metaphocyte progenitor cells |
| Organism |
Danio rerio |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Metaphocytes are tissue-resident myeloid-like cells of non-hematopoietic origin in zebrafish barrier tissues. How metaphocytes acquire myeloid cell properties from non-hematopoietic precursors during the development remains unknown. Here we show that metaphocytes are in situ generated from local progenitors guided by the ETS transcription factor Spic. Tg(spic:EGFP) transgenic fish were found to label both metaphocytes and their progenitor cells. While spic is dispensable for the formation of the metaphocyte progenitors, it is required for the progenitors to differentiate into immature metaphocytes. To decipher the molecular basis underlying the developmental arrest of metaphocytes in spic mutants, we sorted the metaphocyte progenitor cells from the epidermis and gill of the mutants and siblings and performed bulk RNA-seq analysis. The data suggests that Spic orchestrates the development of metaphocytes by promoting the expression of myeloid-associated genes, either directly or indirectly through downstream transcription factors.
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| Overall design |
Gene expression profiling analysis of bulk RNA-seq data for metaphocyte progenitor cells from the epidermis and gill of spic mutant fish and siblings
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| Contributor(s) |
Zhao C, Li Y, Tang J, Zhou Q, Lin X, Wen Z |
| Citation(s) |
37148242 |
| Submission date |
Apr 02, 2023 |
| Last update date |
Jul 08, 2023 |
| Contact name |
Zilong WEN |
| E-mail(s) |
zilong@ust.hk
|
| Phone |
+852 23587291
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| Organization name |
Hong Kong University of Science and Technology
|
| Department |
Life Science
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| Lab |
WEN lab
|
| Street address |
Rm6319, HKUST, Clear water bay, Kowloon
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| City |
Hong Kong |
| ZIP/Postal code |
000000 |
| Country |
Hong Kong |
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| Platforms (1) |
| GPL24995 |
Illumina NovaSeq 6000 (Danio rerio) |
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| Samples (27)
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| This SubSeries is part of SuperSeries: |
| GSE228748 |
Metaphocytes Are IL-22BP Producing Cells Regulated by ETS Transcription Factor Spic and Essential for Zebrafish Barrier Immunity |
|
| Relations |
| BioProject |
PRJNA951376 |
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