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| Status |
Public on Mar 23, 2021 |
| Title |
A Non-Canonical Type 2 Immune Response Coordinates Tuberculous Granuloma Formation and Epithelialization |
| Organism |
Danio rerio |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Using the zebrafish-M. marinum model, we identify the basis of granuloma macrophage transformation. Single-cell RNA-seq analysis of zebrafish granulomas as well as analysis of M. tuberculosis-infected macaques reveal that, even in the presence of robust type 1 immune responses, countervailing type 2 signals associate with macrophage epithelialization. We find that type 2 immune signaling, mediated via stat6, is absolutely required for epithelialization and granuloma formation.
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| |
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| Overall design |
Wildtype zebrafish-M. marinum granuloma single-cell RNAseq profile.
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| Contributor(s) |
Hughes EJ, Cronan MR, Gregory SG, Tobin DM |
| Citation(s) |
33761328 |
| |
| Submission date |
Nov 18, 2020 |
| Last update date |
Mar 26, 2021 |
| Contact name |
David M Tobin |
| E-mail(s) |
david.tobin@duke.edu
|
| Phone |
919-684-9152
|
| Organization name |
Duke University
|
| Department |
Molecular Genetics and Microbiology
|
| Lab |
Tobin
|
| Street address |
304 Research Drive, 207A Jones Building
|
| City |
Durham |
| State/province |
NC |
| ZIP/Postal code |
27710 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL20828 |
Illumina NextSeq 500 (Danio rerio) |
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| Samples (1) |
| GSM4913139 |
*AB wildtype zebrafish-M.marinum granuloma cells |
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| Relations |
| BioProject |
PRJNA679243 |
| SRA |
SRP293088 |
