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| Status |
Public on Jan 17, 2020 |
| Title |
Expression of formate-THF ligase has a deep impact on primary metabolism of Synechocystis sp. PCC 6803 |
| Organism |
Synechocystis sp. PCC 6803 |
| Experiment type |
Expression profiling by array
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| Summary |
The introduction of alternative CO2-fixing pathways such as formate synthesis and assimilation may improve the efficiency of biological carbon fixation that appears to be limited by the enzymatic properties of ribulose 1,5-bisphosphate carboxylase/oxygenase (RubisCO). Here we aimed to establish a formate assimilation pathway in the model cyanobacterium Synechocystis sp. PCC 6803. The formate-tetrahydrofolate ligase (FTL) from Methylobacterium extorquens AM1 was expressed in Synechocystis to enable formate assimilation and reduce the loss of fixed carbon in the photorespiratory pathway. Transgenic strains accumulated serine and 3-phosphoglycerate, and consumed more 2-phosphoglycolate and glycine, which seemed to reflect the efficient utilization of formate. However, labelling experiments showed that the serine accumulation was not due to the expected incorporation of formate. DNA-microarray experiments were performed to analyze possible transcriptome changes due to ftl expression. Marked changes in expression of genes encoding proteins associated with serine biosynthesis and enzymes involved in nitrogen and C1 metabolism revealed that ftl expression had a regulatory impact on these metabolic routes. Our results indicate that the expression of new pathways could have a severe impact on the cellular regulatory network, which hampers the establishment of newly designed pathways.
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| Overall design |
Synechocstis PCC 6803 WT cells and cells expressing formyl-THF ligase (FTL) from Methylobacterium extorquens AM1 were studied in the presence of absence of 10 mM sodium formate for 3 days under constant illumination and air bubbling. Samples were taken in biological replicates.
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| Contributor(s) |
Song S, Timm S, Linder SN, Reimann V, Hess WR, Hagemann M, Brouwer E |
| Citation(s) |
32760387 |
| Submission date |
Jan 16, 2020 |
| Last update date |
Aug 10, 2020 |
| Contact name |
Wolfgang R. Hess |
| E-mail(s) |
wolfgang.hess@biologie.uni-freiburg.de
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| Phone |
49-761-2032796
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| Organization name |
University of Freiburg
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| Department |
Institute of Biology III
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| Lab |
Genetics & Experimental Bioinformatics
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| Street address |
Schänzlestrasse 1
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| City |
Freiburg |
| State/province |
Baden-Würtemberg |
| ZIP/Postal code |
79104 |
| Country |
Germany |
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| Platforms (1) |
| GPL23938 |
Agilent-075764 Synechocystis sp. PCC 6803 array (Feature number version) |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA601679 |
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