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| Status |
Public on Mar 06, 2019 |
| Title |
Single cell transcriptome analysis of TSCs differentiated TSCs, pTSCs and pTSCs isolated from blastoid |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
We performed scRNA seq according to the CEL-seq2 protocol in order to define the equivalent developmental stages of TSCs
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| Overall design |
Using FACS, we sorted single cells into 384 well plates for TSCs derived in the Rossant lab. TSCs are cultured in Tx conditions. Differentiated TSCs have undergone differentiation after 6 days in culture without Fgf4, Tgfb nor heparin. pTSCs are culture din LT21 conditions. Blastoid single cells are isolated from harvested blastoids formed using pTS1 pTSCs.
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| Contributor(s) |
Frias Aldeguer J, Rivron N, Vivié J, Alemany Arias A, Geijsen N, van Blitterswijk C |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Mar 03, 2019 |
| Last update date |
Mar 25, 2019 |
| Contact name |
Nicolas Rivron |
| Phone |
+31302121800
|
| Organization name |
Hubrecht Institute / Maastricht University
|
| Lab |
Rivron
|
| Street address |
Uppsalalaan 8
|
| City |
Utrecht |
| State/province |
Utrecht |
| ZIP/Postal code |
3584CT |
| Country |
Netherlands |
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| Platforms (1) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
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| Samples (10)
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| Relations |
| BioProject |
PRJNA525281 |
| SRA |
SRP187355 |
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