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| Status |
Public on Feb 05, 2018 |
| Title |
H3K27 acetylation and gene expression during late spermatogenesis |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
ChIP-seq for H3K27 acetylation and RNA-seq were performed during spermatogenesis. We analyzed two representative stages of spermatogenesis: purified pachytene spermatocytes (PS) undergoing meiosis; and postmeiotic round spermatids (RS) from adult testes.
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| Overall design |
ChIP-seq analyses using spermatocytes and spermatides from wild-type mice and RNA-seq analysis using spermatocytes and spermatides from Rnf8- and Scml2-dKO mice
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| |
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| Contributor(s) |
Maezawa S, Barski A, Namekawa S |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Nov 27, 2017 |
| Last update date |
May 15, 2019 |
| Contact name |
So Maezawa |
| E-mail(s) |
so.maezawa@cchmc.org
|
| Phone |
513-803-1377
|
| Organization name |
Cincinnati Children's Hospital Medical Center
|
| Department |
Reproductive Sciences
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| Lab |
Namekawa lab
|
| Street address |
3333 Burnet Ave.
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| City |
Cincinnati |
| ZIP/Postal code |
45229 |
| Country |
USA |
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| Platforms (1) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA420064 |
| SRA |
SRP125760 |
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