DAT Dopamine transporter [Drosophila melanogaster (fruit fly)] - Gene

Summary

Official Symbol: DATprovided by FlyBase
Official Full Name: Dopamine transporterprovided by FlyBase
Primary source: FLYBASE:FBgn0034136
Locus tag: Dmel_CG8380
See related: AllianceGenome:FB:FBgn0034136
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Drosophila melanogaster
Lineage: Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; Drosophilidae; Drosophila; Sophophora
Also known as: CG8380; dat; Dat; dDat; dDAT; DmDAT; Dmel\CG8380; fmn; fumin; Fumin
Summary: Enables cocaine binding activity and dopamine:sodium symporter activity. Involved in several processes, including dopamine transport; regulation of presynaptic cytosolic calcium ion concentration; and response to odorant. Is integral component of plasma membrane. Is expressed in several structures, including central nervous system; dopaminergic neurons; ectoderm anlage; ventral sensory complex primordium; and yolk nucleus. Used to study attention deficit hyperactivity disorder and autism spectrum disorder. Human ortholog(s) of this gene implicated in attention deficit hyperactivity disorder; hypertension; neurocirculatory asthenia; nicotine dependence; and obesity. Orthologous to several human genes including SLC6A2 (solute carrier family 6 member 2). [provided by Alliance of Genome Resources, Apr 2022]
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Genomic context

Location:: 53C7-53C8; 2-80 cM

Exon count:: 8

Annotation release	Status	Assembly	Chr	Location
Release 6.54	current	Release 6 plus ISO1 MT (GCF_000001215.4)	2R	NT_033778.4 (16558567..16565238, complement)
Release 5.57	previous assembly	Release 5 (GCF_000001215.2)	2R	NT_033778.3 (12446072..12452743, complement)

Chromosome 2R - NT_033778.4 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Pharmacological Characterization of Purified Full-Length Dopamine Transporter from Drosophila melanogaster.
Title: Pharmacological Characterization of Purified Full-Length Dopamine Transporter from Drosophila melanogaster.
Dopaminergic Ric GTPase activity impacts amphetamine sensitivity and sleep quality in a dopamine transporter-dependent manner in Drosophila melanogaster.
Title: Dopaminergic Ric GTPase activity impacts amphetamine sensitivity and sleep quality in a dopamine transporter-dependent manner in Drosophila melanogaster.
Structural Determinants of the Dopamine Transporter Regulation Mediated by G Proteins.
Title: Structural Determinants of the Dopamine Transporter Regulation Mediated by G Proteins.
Identification of a New Allosteric Binding Site for Cocaine in Dopamine Transporter.
Title: Identification of a New Allosteric Binding Site for Cocaine in Dopamine Transporter.
Structural basis of norepinephrine recognition and transport inhibition in neurotransmitter transporters.
Title: Structural basis of norepinephrine recognition and transport inhibition in neurotransmitter transporters.
Study identified Na+- and dopamine-induced changes in specific regions of the wild-type Drosophila melanogaster dopamine transporter, suggesting their involvement in protein conformational transitions. Ligand-dependent slow cooperative fluctuations of helical stretches in several domains of the transporter were detected, which could be a molecular mechanism that assists in the transporter function.
Title: Substrate-induced conformational dynamics of the dopamine transporter.
When DAT, a member of the post-feeding reward system, is either overexpressed or reduced via mutation, Drosophila has increased sensitivity to amino acid starvation.
Title: Manipulation of components that control feeding behavior in Drosophila melanogaster increases sensitivity to amino acid starvation.
X-ray crystal structures of dopamine transporter (DAT) bound to its substrate dopamine, a substrate analogue 3,4-dichlorophenethylamine, the psychostimulants d-amphetamine and methamphetamine, or to cocaine and cocaine analogues
Title: Neurotransmitter and psychostimulant recognition by the dopamine transporter.
Phosphatidylinositol-4,5-Biphosphate regulates psychostimulant behaviors through its interaction with a membrane dopamine transporter.
Title: PIP2 regulates psychostimulant behaviors through its interaction with a membrane protein.
the dopamine transporter X ray structure reveals the molecular basis for antidepressant action on sodium-coupled neurotransmitter symporters and elucidates critical elements of eukaryotic transporter structure and modulation by lipids
Title: X-ray structure of dopamine transporter elucidates antidepressant mechanism.

Submit: New GeneRIF Correction See all GeneRIFs (12)

Pathways from PubChem

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General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by FlyBase

Function	Evidence Code	Pubs
enables cocaine binding	IDA Inferred from Direct Assay more info	PubMed
enables dopamine:sodium symporter activity	IBA Inferred from Biological aspect of Ancestor more info
enables dopamine:sodium symporter activity	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
involved_in adult locomotory behavior	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in amino acid transport	IBA Inferred from Biological aspect of Ancestor more info
involved_in circadian sleep/wake cycle	IDA Inferred from Direct Assay more info	PubMed
involved_in circadian sleep/wake cycle	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in dopamine transport	IDA Inferred from Direct Assay more info	PubMed
involved_in dopamine uptake involved in synaptic transmission	IBA Inferred from Biological aspect of Ancestor more info
involved_in norepinephrine transport	IBA Inferred from Biological aspect of Ancestor more info
involved_in regulation of presynaptic cytosolic calcium ion concentration	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in response to odorant	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in sleep	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in sodium ion transmembrane transport	IBA Inferred from Biological aspect of Ancestor more info

Component	Evidence Code	Pubs
is_active_in axon	IBA Inferred from Biological aspect of Ancestor more info
located_in membrane	IEA Inferred from Electronic Annotation more info
is_active_in neuronal cell body membrane	IBA Inferred from Biological aspect of Ancestor more info
is_active_in plasma membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in plasma membrane	IDA Inferred from Direct Assay more info	PubMed
located_in plasma membrane	ISM Inferred from Sequence Model more info	PubMed
is_active_in presynaptic membrane	IBA Inferred from Biological aspect of Ancestor more info

General protein information

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Preferred Names: dopamine transporter

Names: CG8380-PA; CG8380-PB; DAT-PA; DAT-PB; fumin

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NT_033778.4 Reference assembly

Range

16558567..16565238 complement

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_079039.4 → NP_523763.2 dopamine transporter, isoform A [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_523763.2

Status: REVIEWED

UniProtKB/Swiss-Prot

Q7K4Y6, Q9NB97

Related

FBpp0086271

Conserved Domains (1) summary

cd11556
Location:27 → 597

SLC6sbd_SERT-like_u1; uncharacterized subgroup of the SERT-like Na(+)- and Cl(-)-dependent monoamine transporter subfamily; solute binding domain
NM_001274097.2 → NP_001261026.1 dopamine transporter, isoform B [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_001261026.1

Status: REVIEWED

UniProtKB/TrEMBL

A0A0B4KEX2

Conserved Domains (1) summary

cd11556
Location:27 → 597

SLC6sbd_SERT-like_u1; uncharacterized subgroup of the SERT-like Na(+)- and Cl(-)-dependent monoamine transporter subfamily; solute binding domain