THIC thiaminC [Arabidopsis thaliana (thale cress)] - Gene

Summary

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Gene symbol: THIC
Gene description: thiaminC
Primary source: Araport:AT2G29630
Locus tag: AT2G29630
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Arabidopsis thaliana (ecotype: Columbia)
Lineage: Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
Also known as: PY; PYRIMIDINE REQUIRING; T27A16.27; T27A16_27; thiaminC
Summary: Encodes a protein involved in thiamin biosynthesis. The protein is an iron-sulfur cluster protein predicted to catalyze the conversion of 5-aminoimidazole ribonucleotide (AIR) to hydroxymethylpyrimidine (HMP) or hydroxymethylpyrimidine phosphate (HMP-P). A severe reduction of THIC levels in plants decreases vitamin B1 (thiamin diphosphate (TPP)) levels and also leads to changes in the levels of numerous other metabolites since so many primary metabolic enzymes require a TPP co-factor. thiC mutants are chlorotic and arrest in their development at the cotyledon stage. A N-terminal targeting sequence directs the THIC protein to the chloroplast stroma. A conserved TPP-binding site is located in the 3' UTR of the At2g29630.2 gene model, and is predicted to function as a riboswitch. The riboswitch controls the formation of transcripts with alternative 3' UTR lengths, which affect mRNA accumulation and protein production. THIC transcripts are observed in seedlings 5 or more days after germination, and light promotes the expression of this gene. Recessive mutant isolated by Redei. Leaves but not cotyledons white, lethal; restored to normal by thiamine or 2,5-dimethyl-4-aminopyrimidine.
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Genomic context

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Location:: chromosome: 2

Exon count:: 7

Sequence:: Chromosome: 2; NC_003071.7 (12666935..12671055)

Chromosome 2 - NC_003071.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_003071.7

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Metabolic engineering provides insight into the regulation of thiamin biosynthesis in plants.
Title: Metabolic engineering provides insight into the regulation of thiamin biosynthesis in plants.
the expression of TenA_E and ThiC genes in Arabidopsis and maize was inversely correlated.
Title: Salvage of the thiamin pyrimidine moiety by plant TenA proteins lacking an active-site cysteine.
Thic protein crystal structure shows a metal ion with multiple coordinated water molecules in the close vicinity of the substrate binding sites.
Title: High-resolution crystal structure of the eukaryotic HMP-P synthase (THIC) from Arabidopsis thaliana.
In Arabidopsis, the THIC promoter and the thiamin-pyrophosphate riboswitch act simultaneously to tightly regulate thiamin biosynthesis in a circadian manner and consequently sense and control vital points of core cellular metabolism.
Title: Orchestration of thiamin biosynthesis and central metabolism by combined action of the thiamin pyrophosphate riboswitch and the circadian clock in Arabidopsis.
The results suggest that vitamin B1 biosynthesis in plants is in fact more similar to prokaryotic counterparts and that the THIC protein is likely to be the key regulatory protein in the pathway.
Title: Vitamin B1 biosynthesis in plants requires the essential iron sulfur cluster protein, THIC.

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Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

NM_001202705.1

Supported by ESTs:

NM_001336219.1

Supported by ESTs:

NM_128517.4

Supported by ESTs:

NM_179804.3

Supported by ESTs:

Gene Ontology Provided by TAIR

Function	Evidence Code	Pubs
enables ADP-ribose pyrophosphohydrolase activity	IGI Inferred from Genetic Interaction more info	PubMed
enables carbon-carbon lyase activity	IEA Inferred from Electronic Annotation more info
enables iron-sulfur cluster binding	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
acts_upstream_of_or_within detection of bacterium	IMP Inferred from Mutant Phenotype more info
acts_upstream_of_or_within response to vitamin B1	IEP Inferred from Expression Pattern more info	PubMed
acts_upstream_of_or_within thiamine biosynthetic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in thiamine diphosphate biosynthetic process	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
located_in chloroplast	HDA more info	PubMed
located_in chloroplast	IDA Inferred from Direct Assay more info	PubMed
located_in chloroplast	ISM Inferred from Sequence Model more info
located_in chloroplast stroma	HDA more info	PubMed
located_in chloroplast stroma	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	ISM Inferred from Sequence Model more info
located_in plastid	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: thiaminC

NP_001189634.1

thiaminC (THIC); CONTAINS InterPro DOMAIN/s: Thiamine biosynthesis protein ThiC (InterPro:IPR002817).

NP_180524.1

thiaminC (THIC); FUNCTIONS IN: iron-sulfur cluster binding, catalytic activity, ADP-ribose pyrophosphohydrolase activity; INVOLVED IN: response to vitamin B1, detection of bacterium, thiamin biosynthetic process; LOCATED IN: chloroplast, chloroplast stroma, plastid; EXPRESSED IN: 28 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: Thiamine biosynthesis protein ThiC (InterPro:IPR002817); Has 4416 Blast hits to 4411 proteins in 1719 species: Archae - 238; Bacteria - 3466; Metazoa - 3; Fungi - 0; Plants - 61; Viruses - 0; Other Eukaryotes - 648 (source: NCBI BLink).

NP_850135.1

thiaminC (THIC); FUNCTIONS IN: iron-sulfur cluster binding, catalytic activity, ADP-ribose pyrophosphohydrolase activity; INVOLVED IN: response to vitamin B1, detection of bacterium, thiamin biosynthetic process; LOCATED IN: chloroplast stroma, chloroplast, plastid; EXPRESSED IN: 28 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: Thiamine biosynthesis protein ThiC (InterPro:IPR002817); Has 4416 Blast hits to 4411 proteins in 1719 species: Archae - 238; Bacteria - 3466; Metazoa - 3; Fungi - 0; Plants - 61; Viruses - 0; Other Eukaryotes - 648 (source: NCBI BLink).