LAS17 actin-binding protein LAS17 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Gene symbol: LAS17
Gene description: actin-binding protein LAS17
Primary source: SGD:S000005707
Locus tag: YOR181W
See related: AllianceGenome:SGD:S000005707
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as: BEE1
Summary: Enables Arp2/3 complex binding activity and actin binding activity. Involved in actin cortical patch localization; actin filament organization; and positive regulation of supramolecular fiber organization. Located in actin cortical patch. Part of SLAC complex. Used to study Wiskott-Aldrich syndrome. Human ortholog(s) of this gene implicated in Wiskott-Aldrich syndrome; X-linked severe congenital neutropenia; and thrombocytopenia. Orthologous to several human genes including WAS (WASP actin nucleation promoting factor). [provided by Alliance of Genome Resources, Apr 2022]
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Genomic context

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Location:: chromosome: XV

Exon count:: 1

Sequence:: Chromosome: XV; NC_001147.6 (675939..677840)

Chromosome XV - NC_001147.6 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001147.6

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

These studies provide evidence that WASP (Las17) and WIP (Vrp1) play central roles in establishment of a robust multivalent Src homology 3 domain-proline-rich motif network in vivo, giving Actin-related protein 2/3 complex assembly onset at endocytic sites a switch-like behavior.
Title: Switch-like Arp2/3 activation upon WASP and WIP recruitment to an apparent threshold level by multivalent linker proteins in vivo.
Studies indicate that the WASP homologue Las17 is able to nucleate actin independently of Arp2/3 through the function of G-actin-binding activities in its polyproline region.
Title: WASP family proteins, more than Arp2/3 activators.
A model is proposed in which actin filaments bind Vps1 during invagination, and this interaction is important to transduce the force of actin polymerization to the membrane to drive successful scission.
Title: A dynamin-actin interaction is required for vesicle scission during endocytosis in yeast.
LGM has relatively lower potency compared to the previously known Las17 G-actin-binding motif, WH2.
Title: A second Las17 monomeric actin-binding motif functions in Arp2/3-dependent actin polymerization during endocytosis.
Lsb1 and/or Lsb2 full-length proteins inhibit Las17-mediated actin polymerization
Title: Lsb1 is a negative regulator of las17 dependent actin polymerization involved in endocytosis.
polyproline domain able to nucleate actin filaments independently of Arp2/3
Title: A novel actin-binding motif in Las17/WASP nucleates actin filaments independently of Arp2/3.
ARPC1 performs propagation of WASp activation signals via contacts with p19/ARPC2 and direct facilitation of actin nucleation through interactions of the extended arm with the VCA domain of WASp
Title: The p40/ARPC1 subunit of Arp2/3 complex performs multiple essential roles in WASp-regulated actin nucleation.
Results identify an N-terminal fragment of Las17p able to interact with Vrp1p but not Arp2/3, and suggest that LAs17 interaction with Vrp1 is more important than interaction with Arp2/3 for polarized actin assembly and endocytosis in yeast.
Title: Las17p-Vrp1p but not Las17p-Arp2/3 interaction is important for actin patch polarization in yeast.
polyproline region of Las17 and Ysc84 can promote actin polymerization
Title: The WASP homologue Las17 activates the novel actin-regulatory activity of Ysc84 to promote endocytosis in yeast.
Findings highlight the role of Las17p binding for Vrp1p membrane association, suggest general membrane association may be more important than specific targeting to the cortical actin cytoskeleton for Vrp1p. function in endocytosis and cell growth.
Title: Verprolin function in endocytosis and actin organization. Roles of the Las17p (yeast WASP)-binding domain and a novel C-terminal actin-binding domain.

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Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables Arp2/3 complex binding	IDA Inferred from Direct Assay more info	PubMed
enables actin binding	IDA Inferred from Direct Assay more info	PubMed
enables actin binding	IEA Inferred from Electronic Annotation more info
enables actin binding	IMP Inferred from Mutant Phenotype more info	PubMed
enables actin binding	IPI Inferred from Physical Interaction more info	PubMed
enables small GTPase binding	IBA Inferred from Biological aspect of Ancestor more info

Process	Evidence Code	Pubs
involved_in actin cortical patch localization	IGI Inferred from Genetic Interaction more info	PubMed
involved_in actin cortical patch localization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in actin filament organization	IDA Inferred from Direct Assay more info	PubMed
involved_in actin filament polymerization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in actin nucleation	IDA Inferred from Direct Assay more info	PubMed
involved_in positive regulation of Arp2/3 complex-mediated actin nucleation	IDA Inferred from Direct Assay more info	PubMed
involved_in positive regulation of actin filament bundle assembly	IDA Inferred from Direct Assay more info	PubMed
involved_in regulation of actin filament polymerization	IBA Inferred from Biological aspect of Ancestor more info

Component	Evidence Code	Pubs
part_of SLAC complex	IDA Inferred from Direct Assay more info	PubMed
part_of SLAC complex	IPI Inferred from Physical Interaction more info	PubMed
located_in actin cortical patch	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	HDA more info	PubMed
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in mating projection tip	HDA more info	PubMed

General protein information

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Preferred Names: actin-binding protein LAS17

NP_014824.1

Actin assembly factor; C-terminal WCA domain activates Arp2/3 complex-mediated nucleation of branched actin filaments, polyproline domain nucleates actin filaments independent of Arp2/3; mutants are defective in endocytosis, bud site selection, cytokinesis; human homolog WAS (Wiskott-Aldrich Syndrome) implicated in severe immunodeficiency; human WAS complements yeast null mutant, but only in presence of WIPF1, which mediates localization of WAS to cortical patches

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001147.6 Reference assembly

Range

675939..677840

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001183600.1 → NP_014824.1 TPA: actin-binding protein LAS17 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_014824.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6W2N7, Q12446

UniProtKB/TrEMBL

A0A8H4BVX7, B3LJL2, N1NYK6

Conserved Domains (1) summary

cd01205
Location:23 → 123

EVH1_WASP-like; WASP family proteins EVH1 domain