rapA RNA polymerase-binding ATPase and RNAP recycling factor [Escherichia coli str. K-12 substr. MG1655] - Gene

Summary

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Gene symbol: rapA
Gene description: RNA polymerase-binding ATPase and RNAP recycling factor
Primary source: ASAP:ABE-0000198
Locus tag: b0059
See related: ECOCYC:EG11083
Gene type: protein coding
RefSeq status: PROVISIONAL
Organism: Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage: Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as: ECK0060; hepA; yabA
Summary: HepA has sequence similarity to SWI2/SNF2 helicases. hepA is downstream of polB, but is not under lexA control. [More information is available at EcoGene: EG11083]. HepA causes increased recycling of RNA Polymerase and activates transcription under conditions under which the DNA is expected to be tightly supercoiled . [More information is available at EcoCyc: EG11083].
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Genomic context

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Sequence:: NC_000913.3 (60358..63264, complement)

NC_000913.3 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

On the stability of stalled RNA polymerase and its removal by RapA.
Title: On the stability of stalled RNA polymerase and its removal by RapA.
Structural basis of RNA polymerase recycling by the Swi2/Snf2 family of ATPase RapA in Escherichia coli.
Title: Structural basis of RNA polymerase recycling by the Swi2/Snf2 family of ATPase RapA in Escherichia coli.
Structural basis for activation of Swi2/Snf2 ATPase RapA by RNA polymerase.
Title: Structural basis for activation of Swi2/Snf2 ATPase RapA by RNA polymerase.
biochemical properties of RapA and role in RNAP recycling during transcription [review]
Title: Structure and function of RapA: a bacterial Swi2/Snf2 protein required for RNA polymerase recycling in transcription.
Mutations in the interface between RapA's SWI/SNF family chromatin remodeling complex and double-stranded nucleic acid-binding domains significantly alter adenosine triphosphate (ATP) hydrolysis in purified RapA.
Title: RapA, the SWI/SNF subunit of Escherichia coli RNA polymerase, promotes the release of nascent RNA from transcription complexes.
RapA binds and remodels RNA during transcription.
Title: Escherichia coli RNA polymerase-associated SWI/SNF protein RapA: evidence for RNA-directed binding and remodeling activity.
A comparison of the biofilm matrix of the wild-type and rapA strains revealed decreased polysaccharide quantities and coverage in the mutant biofilms
Title: Role of the rapA gene in controlling antibiotic resistance of Escherichia coli biofilms.
N-terminus verified by Edman degradation on mature peptide
Title: RapA, a bacterial homolog of SWI2/SNF2, stimulates RNA polymerase recycling in transcription.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Gene Ontology Provided by EcoCyc

Function	Evidence Code	Pubs
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables ATP hydrolysis activity	IDA Inferred from Direct Assay more info	PubMed
enables ATP-dependent chromatin remodeler activity	IEA Inferred from Electronic Annotation more info
enables DNA binding	IEA Inferred from Electronic Annotation more info
enables bacterial-type RNA polymerase core enzyme binding	IDA Inferred from Direct Assay more info	PubMed
enables helicase activity	IEA Inferred from Electronic Annotation more info
enables hydrolase activity	IEA Inferred from Electronic Annotation more info
enables hydrolase activity, acting on acid anhydrides	IEA Inferred from Electronic Annotation more info
enables nucleic acid binding	IDA Inferred from Direct Assay more info	PubMed
enables nucleotide binding	IEA Inferred from Electronic Annotation more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
involved_in chromatin remodeling	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within positive regulation of DNA-templated transcription	IDA Inferred from Direct Assay more info	PubMed
involved_in regulation of DNA-templated transcription	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: RNA polymerase-binding ATPase and RNAP recycling factor

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_000913.3 Reference assembly

Range

60358..63264 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NP_414601.1 RNA polymerase-binding ATPase and RNAP recycling factor [Escherichia coli str. K-12 substr. MG1655]

See identical proteins and their annotated locations for NP_414601.1

Status: PROVISIONAL

UniProtKB/TrEMBL

A0A3Y4XU34, A0A775USF2, A0A7A6VZE1, A0A7D7KEX3, A0AA87EY90

Conserved Domains (1) summary

PRK04914
Location:2 → 966

PRK04914; RNA polymerase-associated protein RapA