IRX14 Nucleotide-diphospho-sugar transferases superfamily protein [Arabidopsis thaliana (thale cress)] - Gene

Summary

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Gene symbol: IRX14
Gene description: Nucleotide-diphospho-sugar transferases superfamily protein
Primary source: Araport:AT4G36890
Locus tag: AT4G36890
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Arabidopsis thaliana (ecotype: Columbia)
Lineage: Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
Also known as: AP22.51; AP22_51; irregular xylem 14
Summary: The IRX14 gene encodes a putative family 43 glycosyl transferase that contributes to xylan biosynthesis. It was identified based on its gene expression co-variance with the IRX3 gene involved in secondary cell wall synthesis. A biochemical assay using the irx14 mutant indicates that IRX14 might function in xylose chain elongation.
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Genomic context

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Location:: chromosome: 4

Exon count:: 3

Sequence:: Chromosome: 4; NC_003075.7 (17379173..17382028, complement)

Chromosome 4 - NC_003075.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_003075.7

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Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

IRX14 was required for the biosynthesis of seed mucilage xylan.
Title: Xylan synthesized by Irregular Xylem 14 (IRX14) maintains the structure of seed coat mucilage in Arabidopsis.
MUCI21, a member of an uncharacterized clade of the GT61 family, and IRX14 (GT43 protein) are essential for the synthesis of highly branched xylan in seed coat epidermal cells.
Title: Highly Branched Xylan Made by IRREGULAR XYLEM14 and MUCILAGE-RELATED21 Links Mucilage to Arabidopsis Seeds.
The putative glycosyltransferases IRX9, IRX14, and IRX10 (or the paralogs IRX9L, IRX14L, and IRX10L) are required for xylan backbone synthesis in Arabidopsis.
Title: Site-directed mutagenesis of IRX9, IRX9L and IRX14 proteins involved in xylan biosynthesis: glycosyltransferase activity is not required for IRX9 function in Arabidopsis.
The enzymatic activities of two Arabidopsis thaliana GT43 members, IRX9 (Irregular Xylem9) and IRX14, were investigated.
Title: Arabidopsis family GT43 members are xylan xylosyltransferases required for the elongation of the xylan backbone.
IRX14L likely functions redundantly with IRX14 in glucuronoxylan biosynthesis, with IRX14 having a more important role in the process.
Title: IRX14 and IRX14-LIKE, two glycosyl transferases involved in glucuronoxylan biosynthesis and drought tolerance in Arabidopsis.
Double mutations of IRX9 and IRX14 were shown to cause a loss of secondary wall thickening in fibers and a much more severe reduction in glucuronoxylan synthesis.
Title: The Arabidopsis family GT43 glycosyltransferases form two functionally nonredundant groups essential for the elongation of glucuronoxylan backbone.

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Pathways from PubChem

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General gene information

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Homology

Orthologs from OrthoDB

NM_119853.4

Supported by ESTs:

Gene Ontology Provided by TAIR

Function	Evidence Code	Pubs
enables 1,4-beta-D-xylan synthase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase activity	IEA Inferred from Electronic Annotation more info
enables glycosyltransferase activity	ISS Inferred from Sequence or Structural Similarity more info
enables xylosyltransferase activity	IMP Inferred from Mutant Phenotype more info	PubMed

Process	Evidence Code	Pubs
acts_upstream_of_or_within fruit development	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within glucuronoxylan biosynthetic process	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within mucilage biosynthetic process involved in seed coat development	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in mucilage extrusion from seed coat	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within mucilage pectin biosynthetic process	IGI Inferred from Genetic Interaction more info	PubMed
involved_in mucilage pectin biosynthetic process	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within rhamnogalacturonan I biosynthetic process	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within shoot system development	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within xylan biosynthetic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in xylan metabolic process	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within xylem and phloem pattern formation	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in Golgi apparatus	HDA more info	PubMed
located_in Golgi apparatus	IDA Inferred from Direct Assay more info	PubMed
located_in Golgi apparatus	ISM Inferred from Sequence Model more info
located_in Golgi trans cisterna	HDA more info	PubMed
located_in Golgi trans cisterna	IDA Inferred from Direct Assay more info	PubMed
located_in endosome	HDA more info	PubMed
located_in trans-Golgi network	HDA more info	PubMed

General protein information

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Preferred Names: Nucleotide-diphospho-sugar transferases superfamily protein

NP_195407.2

irregular xylem 14 (IRX14); CONTAINS InterPro DOMAIN/s: Glycosyl transferase, family 43 (InterPro:IPR005027); BEST Arabidopsis thaliana protein match is: Nucleotide-diphospho-sugar transferases superfamily protein (TAIR:AT5G67230.1); Has 636 Blast hits to 635 proteins in 91 species: Archae - 0; Bacteria - 2; Metazoa - 399; Fungi - 0; Plants - 221; Viruses - 0; Other Eukaryotes - 14 (source: NCBI BLink).

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_003075.7 Reference assembly

Range

17379173..17382028 complement

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_119853.4 → NP_195407.2 Nucleotide-diphospho-sugar transferases superfamily protein [Arabidopsis thaliana]

See identical proteins and their annotated locations for NP_195407.2

Status: REVIEWED

UniProtKB/Swiss-Prot

O23194, Q8L707

UniProtKB/TrEMBL

A0A178UUK1, A0A384KJH8, A0A654FW89, W8Q2W9

Conserved Domains (1) summary

cl11394
Location:160 → 428

Glyco_tranf_GTA_type; Glycosyltransferase family A (GT-A) includes diverse families of glycosyl transferases with a common GT-A type structural fold