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    CHLI1 P-loop containing nucleoside triphosphate hydrolases superfamily protein [ Arabidopsis thaliana (thale cress) ]

    Gene ID: 827580, updated on 7-Sep-2023

    Summary

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    Gene symbol
    CHLI1
    Gene description
    P-loop containing nucleoside triphosphate hydrolases superfamily protein
    Primary source
    Araport:AT4G18480
    Locus tag
    AT4G18480
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Arabidopsis thaliana (ecotype: Columbia)
    Lineage
    Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
    Also known as
    CH-42; CH42; CHL11; CHLI-1; CHLORINA 42; F28J12.140; F28J12_140; LOST1; low temperature with open-stomata 1; PROTOPORPHYRIN-IX MG-CHELATASE
    Summary
    Encodes the CHLI subunit of magnesium chelatase which is required for chlorophyll biosynthesis. All four cysteine residues of the protein form two disulfide bonds (Cys102-Cys193 and Cys354-Cys396) under oxidized conditions but are fully reduced by reduction. It was suggested that the redox state of CHLI is regulated in vivo by the change of the redox environment in the chloroplasts probably via the Trx system.
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    Genomic context

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    Location:
    chromosome: 4
    Exon count:
    3
    Sequence:
    Chromosome: 4; NC_003075.7 (10201672..10203469, complement)

    Chromosome 4 - NC_003075.7Genomic Context describing neighboring genes Neighboring gene Integrase-type DNA-binding superfamily protein Neighboring gene negative regulator of systemic acquired resistance (SNI1) Neighboring gene D-Tyr-tRNA(Tyr) deacylase family protein Neighboring gene RNA helicase family protein Neighboring gene uncharacterized protein Neighboring gene uncharacterized protein Neighboring gene uncharacterized protein

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_003075.7

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. The CHLI1 subunit of Arabidopsis thaliana magnesium chelatase is a target protein of the chloroplast thioredoxin. Ikegami A, et al. J Biol Chem, 2007 Jul 6. PMID 17472958
    2. Isolation of a gene encoding a novel chloroplast protein by T-DNA tagging in Arabidopsis thaliana. Koncz C, et al. EMBO J, 1990 May. PMID 2158442, Free PMC Article
    3. Mg-chelatase I subunit 1 and Mg-protoporphyrin IX methyltransferase affect the stomatal aperture in Arabidopsis thaliana. Tomiyama M, et al. J Plant Res, 2014 Jul. PMID 24840863, Free PMC Article
    4. [Evolution aspects of intraspecific polymorphysm of Arabidopsis thaliana genes coding subunit I of Mg-chelatase complex]. Apchelimov AA, et al. Mol Biol (Mosk), 2009 Sep-Oct. PMID 19899639
    5. Porphyrins promote the association of GENOMES UNCOUPLED 4 and a Mg-chelatase subunit with chloroplast membranes. Adhikari ND, et al. J Biol Chem, 2009 Sep 11. PMID 19605356, Free PMC Article

    See all (20) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. The chlorophyll synthesis enzymes, Mg-chelatase complex and Mg-protoporphyrin IX methyltransferase, specifically affect abscisic acid (ABA) signaling in the control of stomatal aperture and have no effect on ABA-induced gene expression.
      Title: Mg-chelatase I subunit 1 and Mg-protoporphyrin IX methyltransferase affect the stomatal aperture in Arabidopsis thaliana.
    2. Paths of evolutionary dynamics for CHLI 1 and CHLI 2 genes determining different forms of subunit I of Mg-chelatase complex of Arabidopsis thaliana are proposed.
      Title: [Evolution aspects of intraspecific polymorphysm of Arabidopsis thaliana genes coding subunit I of Mg-chelatase complex].
    3. GUN4 stimulating chlorophyll biosynthesis not only by activating Mg-chelatase but also by promoting interactions between ChlH and chloroplast membranes
      Title: Porphyrins promote the association of GENOMES UNCOUPLED 4 and a Mg-chelatase subunit with chloroplast membranes.
    4. CHLI2 could support some chlorophyll biosynthesis in the complete absence of CHLI1.
      Title: Arabidopsis CHLI2 can substitute for CHLI1.
    5. CHLI2 also has ATPase activity but with a lower Vmax and higher Km ATP than CHLI1
      Title: Functional analysis of Arabidopsis thaliana isoforms of the Mg-chelatase CHLI subunit.
    6. Comparison of the amino acid sequence of CHLI 1 and CHLI 2 encoded in the genome of acifluorfen herbicide resistant mutant aci5 and wild type revealed in particular alterations of the C-terminal end.
      Title: The analysis of the ChlI 1 and ChlI 2 genes using acifluorfen-resistant mutant of Arabidopsis thaliana.
    7. Gun4 is involved in the stimulation of Mg-chelatase activity.
      Title: Structure of the Mg-chelatase cofactor GUN4 reveals a novel hand-shaped fold for porphyrin binding.
    Submit: New GeneRIF Correction

    Pathways from PubChem

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    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    NM_117962.3

    Gene Ontology Provided by TAIR

    Function Evidence Code Pubs
    enables ATP hydrolysis activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables magnesium chelatase activity NAS
    Non-traceable Author Statement
    more info
    		 PubMed 
    Process Evidence Code Pubs
    involved_in chlorophyll biosynthetic process IEA
    Inferred from Electronic Annotation
    more info
    		  
    acts_upstream_of_or_within chlorophyll biosynthetic process TAS
    Traceable Author Statement
    more info
    		 PubMed 
    Component Evidence Code Pubs
    located_in chloroplast HDA PubMed 
    located_in chloroplast IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in chloroplast ISM
    Inferred from Sequence Model
    more info
    		  
    located_in chloroplast stroma HDA PubMed 
    located_in chloroplast stroma IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in cytosol HDA PubMed 
    part_of magnesium chelatase complex TAS
    Traceable Author Statement
    more info
    		 PubMed 
    located_in plant-type cell wall HDA PubMed 

    General protein information

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    Preferred Names
    P-loop containing nucleoside triphosphate hydrolases superfamily protein
    NP_193583.1
    • CHLI1; FUNCTIONS IN: magnesium chelatase activity, ATPase activity; INVOLVED IN: chlorophyll biosynthetic process; LOCATED IN: magnesium chelatase complex, cell wall, chloroplast, chloroplast stroma; EXPRESSED IN: 24 plant structures; EXPRESSED DURING: 14 growth stages; CONTAINS InterPro DOMAIN/s: ATPase, AAA+ type, core (InterPro:IPR003593), Magnesium chelatase, ChlI subunit (InterPro:IPR000523), Magnesium chelatase, ATPase subunit I (InterPro:IPR011775); BEST Arabidopsis thaliana protein match is: magnesium chelatase i2 (TAIR:AT5G45930.1); Has 6584 Blast hits to 6581 proteins in 1594 species: Archae - 309; Bacteria - 5009; Metazoa - 2; Fungi - 0; Plants - 206; Viruses - 0; Other Eukaryotes - 1058 (source: NCBI BLink).

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_003075.7 Reference assembly

      Range
      10201672..10203469 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NM_117962.3 → NP_193583.1  P-loop containing nucleoside triphosphate hydrolases superfamily protein [Arabidopsis thaliana]

      See identical proteins and their annotated locations for NP_193583.1

      Status: REVIEWED

      UniProtKB/Swiss-Prot
      P16127, Q0WUK7
      UniProtKB/TrEMBL
      A0A178UXM0, A0A5S9XUB5, A0A7G2F450
      Conserved Domains (1) summary
      CHL00081
      Location:77 → 424
      chlI; Mg-protoporyphyrin IX chelatase
    Nucleotide Protein
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    P16127.1

    Gene LinkOut

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