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rapZ RNase adaptor protein RapZ [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 947727, updated on 2-May-2024

Summary

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Gene symbol
rapZ
Gene description
RNase adaptor protein RapZ
Primary source
ASAP:ABE-0010522
Locus tag
b3205
See related
ECOCYC:EG12146
Gene type
protein coding
RefSeq status
PROVISIONAL
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
ECK3194; yhbJ
Summary
Unprocessed glmZ upregulates glmS mRNA, so yhbJ mediates the negative feedback regulation of glmS, through glmZ, by the GlmS product glucosamine-6-phosphate. [More information is available at EcoGene: EG12146]. YhbJ appears to regulate the expression of GlmS by controlling the processing and stability of the small RNA regulator GlmZ, possibly in response to the intracellular concentration of the product of GlmS, glucosamine-6-phosphate . [More information is available at EcoCyc: EG12146].
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Genomic context

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Sequence:
NC_000913.3 (3347115..3347969)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene ribosome hibernation-promoting factor Neighboring gene phosphotransferase system enzyme IIA(Ntr) Neighboring gene phosphorelay protein NPr Neighboring gene protein kinase-like domain-containing protein YrbL Neighboring gene peptidoglycan glycosyltransferase MtgA Neighboring gene low activity glyoxalase ElbB

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. A Spontaneous rapZ Mutant Impairs Infectivity of Lytic Bacteriophage vB_EcoM_JS09 against Enterotoxigenic Escherichia coli. Zhou Y, et al. mSphere, 2021 Mar 3. PMID 33658278, Free PMC Article
  2. Targeted decay of a regulatory small RNA by an adaptor protein for RNase E and counteraction by an anti-adaptor RNA. Göpel Y, et al. Genes Dev, 2013 Mar 1. PMID 23475961, Free PMC Article
  3. Crystallization and preliminary X-ray diffraction analysis of YhbJ from Escherichia coli, a key protein involved in the GlmYZ sRNA regulatory cascade. Resch M, et al. Acta Crystallogr Sect F Struct Biol Cryst Commun, 2013 Feb 1. PMID 23385747, Free PMC Article
  4. Structure of a bacterial ribonucleoprotein complex central to the control of cell envelope biogenesis. Islam MS, et al. EMBO J, 2023 Jan 16. PMID 36504162, Free PMC Article
  5. Small RNA-binding protein RapZ mediates cell envelope precursor sensing and signaling in Escherichia coli. Khan MA, et al. EMBO J, 2020 Mar 16. PMID 32065419, Free PMC Article

See all (22) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Structure of a bacterial ribonucleoprotein complex central to the control of cell envelope biogenesis.
    Title: Structure of a bacterial ribonucleoprotein complex central to the control of cell envelope biogenesis.
  2. A Spontaneous rapZ Mutant Impairs Infectivity of Lytic Bacteriophage vB_EcoM_JS09 against Enterotoxigenic Escherichia coli.
    Title: A Spontaneous rapZ Mutant Impairs Infectivity of Lytic Bacteriophage vB_EcoM_JS09 against Enterotoxigenic Escherichia coli.
  3. Small RNA-binding protein RapZ mediates cell envelope precursor sensing and signaling in Escherichia coli.
    Title: Small RNA-binding protein RapZ mediates cell envelope precursor sensing and signaling in Escherichia coli.
  4. accumulation of GlmZ* prevents complete GlmZ turnover. This mechanism may serve to adjust a robust glmS basal expression level that is buffered against fluctuations in RapZ availability.
    Title: Feedback regulation of small RNA processing by the cleavage product.
  5. data supports a model in which RapZ forms a complex with GlmZ that interacts with RNase E to guide specific cleavage of the sRNA, thereby down-regulating glmS activity
    Title: Structural insights into RapZ-mediated regulation of bacterial amino-sugar metabolism.
  6. The biological function of GlmZ is to positively control the glmS mRNA in response to GlcN-6-P concentrations and that YhbJ negatively regulates GlmZ. As in yhbJ mutants GlcN-6-P has no effect, YhbJ is essential for sensing this metabolite.
    Title: Feedback control of glucosamine-6-phosphate synthase GlmS expression depends on the small RNA GlmZ and involves the novel protein YhbJ in Escherichia coli.
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General gene information

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Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables ATP binding IEA
Inferred from Electronic Annotation
more info
  
enables GTP binding IEA
Inferred from Electronic Annotation
more info
  
enables RNA binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables RNA binding IEA
Inferred from Electronic Annotation
more info
  
enables carbohydrate derivative binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables identical protein binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables molecular adaptor activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
Process Evidence Code Pubs
acts_upstream_of_or_within RNA destabilization IMP
Inferred from Mutant Phenotype
more info
 PubMed 
acts_upstream_of_or_within protein homotetramerization IDA
Inferred from Direct Assay
more info
 PubMed 
Component Evidence Code Pubs
part_of protein-containing complex IDA
Inferred from Direct Assay
more info
 PubMed 

General protein information

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Preferred Names
RNase adaptor protein RapZ

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    3347115..3347969
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_417672.1 RNase adaptor protein RapZ [Escherichia coli str. K-12 substr. MG1655]

    See identical proteins and their annotated locations for NP_417672.1

    Status: PROVISIONAL

    UniProtKB/TrEMBL
    A0A377AF68, A0A826XUS8
    Conserved Domains (1) summary
    pfam03668
    Location:1 → 282
    ATP_bind_2; P-loop ATPase protein family
Nucleotide Protein
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Gene LinkOut

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Other Literature Sources
Molecular Biology Databases
Research Materials