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    CBS cystathionine-beta-synthase [ Homo sapiens ]

    Gene ID: 875, updated on 20-May-2012

    Summary

    Official Symbol
    CBSprovided by HGNC
    Official Full Name
    cystathionine-beta-synthaseprovided by HGNC
    Primary source
    HGNC:1550
    See related
    Ensembl:ENSG00000160200; HPRD:01994; MIM:613381; Vega:OTTHUMG00000086834
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Homo sapiens
    Lineage
    Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
    Also known as
    HIP4
    Summary
    The protein encoded by this gene acts as a homotetramer to catalyze the conversion of homocysteine to cystathionine, the first step in the transsulfuration pathway. The encoded protein is allosterically activated by adenosyl-methionine and uses pyridoxal phosphate as a cofactor. Defects in this gene can cause cystathionine beta-synthase deficiency (CBSD), which can lead to homocystinuria. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, May 2010]

    Genomic context

    Location :
    21q22.3
    Sequence :
    Chromosome: 21; NC_000021.8 (44473301..44496472, complement)

    Chromosome 21 - NC_000021.8Genomic Context describing neighboring genes Neighboring gene NADH dehydrogenase (ubiquinone) flavoprotein 3, 10kDa Neighboring gene PBX/knotted 1 homeobox 1 Neighboring gene U2 small nuclear RNA auxiliary factor 1 Neighboring gene mitochondrial ribosomal protein L51 pseudogene 2

    Genomic regions, transcripts, and products

    Bibliography

    GeneRIFs: Gene References Into Functions What's a GeneRIF?

    Phenotypes

    Novel associations of CPS1, MUT, NOX4, and DPEP1 with plasma homocysteine in a healthy population: a genome-wide evaluation of 13 974 participants in the Women's Genome Health Study.

    Thrombosis, hyperhomocysteinemic

    Summary from GeneReviews: Go to GeneReviews

    Disease Characteristics
    Homocystinuria caused by cystathionine -synthase (CBS) deficiency is characterized by developmental delay/intellectual disability, ectopia lentis and/or severe myopia, skeletal abnormalities (excessive height and length of the limbs), and thromboembolism. Expressivity is variable for all of the clinical signs. Two phenotypic variants are recognized, B6-responsive homocystinuria and B6-non-responsive homocystinuria. B6-responsive homocystinuria is typically, but not always, milder than the non-responsive variant. In the majority of untreated affected individuals, ectopia lentis occurs by age eight years. Individuals are often tall and slender with an asthenic ( marfanoid ) habitus and are prone to osteoporosis. Thromboembolism is the major cause of early death and morbidity. IQ in individuals with untreated homocystinuria ranges widely, from 10 to 138. In B6-responsive individuals the mean IQ is 79 versus 57 for those who are B6 non-responsive. Other features that may occur include: seizures, psychiatric problems, extrapyramidal signs such as dystonia, hypopigmentation, malar flush, livedo reticularis, and pancreatitis.
    Diagnosis Testing
    The cardinal biochemical features of homocystinuria are: markedly increased concentrations of plasma homocystine, total homocysteine, homocysteine-cysteine mixed disulfide, and methionine; increased concentration of urine homocystine; and reduced cystathionine -synthase (CBS) enzyme activity. Molecular genetic testing of CBS, the gene in which mutations result in homocystinuria caused by cystathionine -synthase deficiency, is clinically available.
    Genetic Counseling
    Homocystinuria is inherited in an autosomal recessive manner. At conception, each sib of an affected individual has a 25% chance of being affected, a 50% chance of being an asymptomatic carrier, and a 25% chance of being unaffected and not a carrier. Carrier testing for at-risk family members is possible once the CBS mutations have been identified in the family. Prenatal testing is possible for fetuses at increased risk through measurement of CBS enzyme activity assayed in cultured amniocytes (but not in chorionic villi because this tissue has very low activity of the CBS enzyme); measurement of total homocysteine in cell-free amniotic fluid; and molecular genetic testing if both disease-causing alleles of an affected family member have been identified.
    References

    Interactions

    Products Interactant Other Gene Complex Source Pubs Description
    P35520 Q8IV36 C17orf28    HPRD  PubMed  
    P35520 P35520 CBS    HPRD  PubMed  
    P35520 P51116 FXR2    HPRD  PubMed  
    P35520 P42858 HTT    HPRD  PubMed  
    P35520 Q9NP79 VTA1    HPRD  PubMed  
    BioGRID:107321 BioGRID:129720 C17orf28    BioGRID  PubMed Two-hybrid 
    BioGRID:107321 BioGRID:107321 CBS    BioGRID  PubMed Affinity Capture-Western; Co-crystal Structure; Two-hybrid 
    BioGRID:107321 BioGRID:114890 FXR2    BioGRID  PubMed Two-hybrid 
    BioGRID:107321 BioGRID:114593 HGS    BioGRID  PubMed Reconstituted Complex 
    BioGRID:107321 BioGRID:32775 HSP26    BioGRID  PubMed Synthetic Rescue 
    BioGRID:107321 BioGRID:111559 PRKAR1A    BioGRID  PubMed Two-hybrid 
    BioGRID:107321 BioGRID:113164 UBC    BioGRID  PubMed Affinity Capture-MS 
    BioGRID:107321 BioGRID:119595 VTA1    BioGRID  PubMed Two-hybrid 

    General gene information

    Markers

    Homology

    • Homologs of the CBS gene: The CBS gene is conserved in chimpanzee, dog, cow, mouse, rat, chicken, zebrafish, fruit fly, mosquito, S.cerevisiae, K.lactis, , , N.crassa, and rice.
    • Map Viewer (Mouse, Rat)

    Pathways from BioSystems

    Gene Ontology Provided by GOA

    Function Evidence Code Pubs
    cystathionine beta-synthase activity IDA
    Inferred from Direct Assay
    more info
    PubMed 
    cystathionine beta-synthase activity TAS
    Traceable Author Statement
    more info
     
    enzyme binding IPI
    Inferred from Physical Interaction
    more info
    PubMed 
    heme binding IDA
    Inferred from Direct Assay
    more info
    PubMed 
    heme binding IMP
    Inferred from Mutant Phenotype
    more info
    PubMed 
    lyase activity IEA
    Inferred from Electronic Annotation
    more info
     
    metal ion binding IEA
    Inferred from Electronic Annotation
    more info
     
    modified amino acid binding IDA
    Inferred from Direct Assay
    more info
     
    protein binding IPI
    Inferred from Physical Interaction
    more info
    PubMed 
    protein homodimerization activity IDA
    Inferred from Direct Assay
    more info
    PubMed 
    pyridoxal phosphate binding IDA
    Inferred from Direct Assay
    more info
    PubMed 
    ubiquitin protein ligase binding IPI
    Inferred from Physical Interaction
    more info
    PubMed 
    Process Evidence Code Pubs
    L-cysteine catabolic process IDA
    Inferred from Direct Assay
    more info
    PubMed 
    L-serine catabolic process IDA
    Inferred from Direct Assay
    more info
    PubMed 
    L-serine metabolic process IDA
    Inferred from Direct Assay
    more info
    PubMed 
    blood vessel remodeling IEA
    Inferred from Electronic Annotation
    more info
     
    cartilage development involved in endochondral bone morphogenesis IEA
    Inferred from Electronic Annotation
    more info
     
    cellular amino acid biosynthetic process IEA
    Inferred from Electronic Annotation
    more info
     
    cellular nitrogen compound metabolic process TAS
    Traceable Author Statement
    more info
     
    cerebellum morphogenesis IEA
    Inferred from Electronic Annotation
    more info
     
    cysteine biosynthetic process from serine IEA
    Inferred from Electronic Annotation
    more info
     
    cysteine biosynthetic process via cystathionine IEA
    Inferred from Electronic Annotation
    more info
     
    endochondral ossification IEA
    Inferred from Electronic Annotation
    more info
     
    homocysteine catabolic process IDA
    Inferred from Direct Assay
    more info
    PubMed 
    homocysteine metabolic process IDA
    Inferred from Direct Assay
    more info
    PubMed 
    hydrogen sulfide biosynthetic process IDA
    Inferred from Direct Assay
    more info
    PubMed 
    hydrogen sulfide biosynthetic process ISS
    Inferred from Sequence or Structural Similarity
    more info
     
    maternal process involved in female pregnancy IEA
    Inferred from Electronic Annotation
    more info
     
    regulation of JUN kinase activity IEA
    Inferred from Electronic Annotation
    more info
     
    regulation of blood vessel size IEA
    Inferred from Electronic Annotation
    more info
     
    regulation of cGMP metabolic process IEA
    Inferred from Electronic Annotation
    more info
     
    response to folic acid IEA
    Inferred from Electronic Annotation
    more info
     
    response to nutrient levels IEA
    Inferred from Electronic Annotation
    more info
     
    small molecule metabolic process TAS
    Traceable Author Statement
    more info
     
    sulfur amino acid metabolic process TAS
    Traceable Author Statement
    more info
     
    superoxide metabolic process IEA
    Inferred from Electronic Annotation
    more info
     
    transsulfuration TAS
    Traceable Author Statement
    more info
     
    Component Evidence Code Pubs
    cytoplasm IEA
    Inferred from Electronic Annotation
    more info
     
    cytosol IDA
    Inferred from Direct Assay
    more info
    PubMed 
    cytosol TAS
    Traceable Author Statement
    more info
     
    intracellular membrane-bounded organelle IDA
    Inferred from Direct Assay
    more info
    PubMed 
    nucleolus IDA
    Inferred from Direct Assay
    more info
    PubMed 
    nucleus IDA
    Inferred from Direct Assay
    more info
    PubMed 
    soluble fraction IEA
    Inferred from Electronic Annotation
    more info
     

    General protein information

    Preferred Names
    cystathionine beta-synthase
    Names
    cystathionine beta-synthase
    beta-thionase
    serine sulfhydrase
    methylcysteine synthase
    NP_000062.1
    NP_001171479.1
    NP_001171480.1

    NCBI Reference Sequences (RefSeq)

    RefSeqs maintained independently of Annotated Genomes

    These reference sequences exist independently of genome builds. Explain

    These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

    Genomic

    1. NG_008938.1 RefSeqGene

      Range
      4569..27740
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NM_000071.2NP_000062.1  cystathionine beta-synthase

      Status: REVIEWED

      Description
      Transcript Variant: This variant (1) and variants 2 and 3 encode the same protein.
      Source sequence(s)
      AL137314, BC000440, DC300040, X82166
      Consensus CDS
      CCDS13693.1
      UniProtKB/Swiss-Prot
      P35520
      UniProtKB/TrEMBL
      Q9NTF0
      Related
      ENSP00000381231, OTTHUMP00000109417, ENST00000398165, OTTHUMT00000195525
      Conserved Domains (3) summary
      cd04608
      Location:419543
      Blast Score: 464
      CBS_pair_PALP_assoc; This cd contains two tandem repeats of the cystathionine beta-synthase (CBS pair) domains associated with the pyridoxal-phosphate (PALP) dependent enzyme domain upstream. The vitamin B6 complex comprises pyridoxine, pyridoxal, and pyridoxamine, as well ...
      cd01561
      Location:85383
      Blast Score: 1174
      CBS_like; CBS_like: This subgroup includes Cystathionine beta-synthase (CBS) and Cysteine synthase. CBS is a unique heme-containing enzyme that catalyzes a pyridoxal 5'-phosphate (PLP)-dependent condensation of serine and homocysteine to give cystathionine. ...
      TIGR01137
      Location:76544
      Blast Score: 2020
      cysta_beta; cystathionine beta-synthase
    2. NM_001178008.1NP_001171479.1  cystathionine beta-synthase

      Status: REVIEWED

      Description
      Transcript Variant: This variant (2) has an alternate 5' UTR exon, as compared to variant 1.
      Source sequence(s)
      AL531222, BC000440, BC007257, BC010242, L19501
      Consensus CDS
      CCDS13693.1
      UniProtKB/Swiss-Prot
      P35520
      Related
      ENSP00000344460, OTTHUMP00000109415, ENST00000352178, OTTHUMT00000195523
      Conserved Domains (3) summary
      cd04608
      Location:419543
      Blast Score: 464
      CBS_pair_PALP_assoc; This cd contains two tandem repeats of the cystathionine beta-synthase (CBS pair) domains associated with the pyridoxal-phosphate (PALP) dependent enzyme domain upstream. The vitamin B6 complex comprises pyridoxine, pyridoxal, and pyridoxamine, as well ...
      cd01561
      Location:85383
      Blast Score: 1174
      CBS_like; CBS_like: This subgroup includes Cystathionine beta-synthase (CBS) and Cysteine synthase. CBS is a unique heme-containing enzyme that catalyzes a pyridoxal 5'-phosphate (PLP)-dependent condensation of serine and homocysteine to give cystathionine. ...
      TIGR01137
      Location:76544
      Blast Score: 2020
      cysta_beta; cystathionine beta-synthase
    3. NM_001178009.1NP_001171480.1  cystathionine beta-synthase

      Status: REVIEWED

      Description
      Transcript Variant: This variant (3) has an alternate 5' UTR exon and lacks an internal segment in the 3' UTR, as compared to variant 1.
      Source sequence(s)
      AL531222, BC000440, BC007257
      Consensus CDS
      CCDS13693.1
      UniProtKB/Swiss-Prot
      P35520
      Related
      ENSP00000352643, OTTHUMP00000109416, ENST00000359624, OTTHUMT00000195524
      Conserved Domains (3) summary
      cd04608
      Location:419543
      Blast Score: 464
      CBS_pair_PALP_assoc; This cd contains two tandem repeats of the cystathionine beta-synthase (CBS pair) domains associated with the pyridoxal-phosphate (PALP) dependent enzyme domain upstream. The vitamin B6 complex comprises pyridoxine, pyridoxal, and pyridoxamine, as well ...
      cd01561
      Location:85383
      Blast Score: 1174
      CBS_like; CBS_like: This subgroup includes Cystathionine beta-synthase (CBS) and Cysteine synthase. CBS is a unique heme-containing enzyme that catalyzes a pyridoxal 5'-phosphate (PLP)-dependent condensation of serine and homocysteine to give cystathionine. ...
      TIGR01137
      Location:76544
      Blast Score: 2020
      cysta_beta; cystathionine beta-synthase

    RefSeqs of Annotated Genomes: Build 37.3

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference GRCh37.p5 Primary Assembly

    Genomic

    1. NC_000021.8 Reference GRCh37.p5 Primary Assembly

      Range
      44473301..44496472, complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    Alternate HuRef

    Genomic

    1. AC_000153.1 Alternate HuRef

      Range
      29891797..29914621, complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

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