PDR16 phosphatidylinositol transporter [Saccharomyces cerevisiae S288C] - Gene

Summary

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Gene symbol: PDR16
Gene description: phosphatidylinositol transporter
Primary source: SGD:S000005175
Locus tag: YNL231C
See related: AllianceGenome:SGD:S000005175
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as: SFH3
Summary: Enables phosphatidylinositol transfer activity and sterol binding activity. Involved in several processes, including negative regulation of sexual sporulation resulting in formation of a cellular spore; phospholipid transport; and sterol biosynthetic process. Located in cell periphery; lipid droplet; and nucleus-vacuole junction. Orthologous to human CLVS2 (clavesin 2). [provided by Alliance of Genome Resources, Apr 2022]
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Genomic context

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Location:: chromosome: XIV

Exon count:: 1

Sequence:: Chromosome: XIV; NC_001146.8 (215987..217042, complement)

Chromosome XIV - NC_001146.8 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001146.8

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Overexpression of a lipid-binding defective mutant of phosphatidylinositol transporter (PDR16) does not confer the resistance to aureobasidin A (AbA), indicating that the lipid-binding activity is essential for acquiring resistance to AbA. In AbA-treated cells, but not in cells in which AUR1 is repressed by the tetracycline-regulatable promoter, the reductions of complex sphingolipid levels are suppressed by the PDR16.
Title: Overexpression of PDR16 confers resistance to complex sphingolipid biosynthesis inhibitor aureobasidin A in yeast Saccharomyces cerevisiae.
The increased susceptibility to azoles, and enhanced changes in sterol biosynthesis upon exposure to azoles of the pdr16Delta strain compared to wt strain, are not the results of increased intracellular concentration of azoles in the pdr16Delta cells.
Title: The yeast Saccharomyces cerevisiae Pdr16p restricts changes in ergosterol biosynthesis caused by the presence of azole antifungals.
The dimeric structure of Sfh3 (Sec14 family homologue 3 in yeast) is reported for the first time and differs from the Sec14 proteins reported to date.
Title: Dimeric Sfh3 has structural changes in its binding pocket that are associated with a dimer-monomer state transformation induced by substrate binding.
Substrate induced dimer-monomer transformation is an essential part of lipid transfer cycles by Sfh3.
Title: Structural determinants for phosphatidylinositol recognition by Sfh3 and substrate-induced dimer-monomer transition during lipid transfer cycles.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables phosphatidylinositol transfer activity	IBA Inferred from Biological aspect of Ancestor more info
enables phosphatidylinositol transfer activity	IDA Inferred from Direct Assay more info	PubMed
enables phosphatidylinositol transfer activity	IGI Inferred from Genetic Interaction more info	PubMed
enables phosphatidylinositol transfer activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables sterol binding	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
involved_in intermembrane lipid transfer	IEA Inferred from Electronic Annotation more info
involved_in lipid transport	IEA Inferred from Electronic Annotation more info
involved_in negative regulation of sexual sporulation resulting in formation of a cellular spore	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in phospholipid biosynthetic process	IGI Inferred from Genetic Interaction more info	PubMed
involved_in phospholipid transport	IDA Inferred from Direct Assay more info	PubMed
involved_in phospholipid transport	IGI Inferred from Genetic Interaction more info	PubMed
involved_in phospholipid transport	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in response to xenobiotic stimulus	IGI Inferred from Genetic Interaction more info	PubMed
involved_in sterol biosynthetic process	IGI Inferred from Genetic Interaction more info	PubMed

Component	Evidence Code	Pubs
located_in cell periphery	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	HDA more info	PubMed
located_in cytosol	HDA more info	PubMed
located_in endoplasmic reticulum	IEA Inferred from Electronic Annotation more info
located_in endoplasmic reticulum membrane	IEA Inferred from Electronic Annotation more info
located_in lipid droplet	IDA Inferred from Direct Assay more info	PubMed
located_in lipid droplet	IEA Inferred from Electronic Annotation more info
located_in membrane	IEA Inferred from Electronic Annotation more info
located_in nucleus-vacuole junction	IDA Inferred from Direct Assay more info	PubMed
located_in plasma membrane	HDA more info	PubMed

General protein information

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Preferred Names: phosphatidylinositol transporter

NP_014168.1

Phosphatidylinositol transfer protein (PITP); controlled by the multiple drug resistance regulator Pdr1p; localizes to lipid particles and microsomes; controls levels of various lipids, may regulate lipid synthesis; homologous to Pdr17p; protein abundance increases in response to DNA replication stress

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001146.8 Reference assembly

Range

215987..217042 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001183069.1 → NP_014168.1 TPA: phosphatidylinositol transporter [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_014168.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6W0W1, P53860

UniProtKB/TrEMBL

A0A6L0ZFZ7, A6ZRL1, B3LP72, B5VQH9, C7GPC8

Conserved Domains (2) summary

pfam00650
Location:142 → 290

CRAL_TRIO; CRAL/TRIO domain

pfam03765
Location:47 → 109

CRAL_TRIO_N; CRAL/TRIO, N-terminal domain