SEC2 guanine nucleotide exchange factor SEC2 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: SEC2
Official Full Name: guanine nucleotide exchange factor SEC2
Primary source: SGD:S000005216
Locus tag: YNL272C
See related: AllianceGenome:SGD:S000005216; FungiDB:YNL272C; VEuPathDB:YNL272C
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary: Enables guanyl-nucleotide exchange factor activity and phosphatidylinositol-4-phosphate binding activity. Involved in ascospore-type prospore membrane formation; autophagy; and exocytosis. Located in several cellular components, including cellular bud neck; cellular bud tip; and transport vesicle. Orthologous to several human genes including RAB3IP (RAB3A interacting protein). [provided by Alliance of Genome Resources, Apr 2022]
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Genomic context

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Location:: chromosome: XIV

Exon count:: 1

Sequence:: Chromosome: XIV; NC_001146.8 (126805..129084, complement)

Chromosome XIV - NC_001146.8 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001146.8

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Hsp110 (Sse1 and Sse2) associates with the 19S regulatory particle of the 26S proteasome and interacts with Hsp70 (Ssa1, Ssa2) to facilitate the delivery of Hsp70 substrates for proteasomal degradation.
Title: Hsp70-Hsp110 chaperones deliver ubiquitin-dependent and -independent substrates to the 26S proteasome for proteolysis in yeast.
Yck1p and Yck2p phosphorylate sites within the Sec2p Ypt32p/Sec15p binding region and in doing so promote binding of Sec2p to Sec15p and inhibit binding to Ypt32p.
Title: The casein kinases Yck1p and Yck2p act in the secretory pathway, in part, by regulating the Rab exchange factor Sec2p.
Structural investigation of the Sec4p-Sec2p complex reveals the role of the Sec2p coiled-coil domain in facilitating the fast kinetics of the exchange reaction.
Title: New insights into the molecular mechanism of the Rab GTPase Sec4p activation.
The phosphorylation state of Sec2p can direct a switch in its regulatory binding partners that facilitates maturation of the secretory vesicle and helps to promote the directionality of vesicular transport.
Title: Phosphorylation of the Rab exchange factor Sec2p directs a switch in regulatory binding partners.
Data suggest that following autophagy induction the function of Sec2 and Sec4 are diverted to direct membrane flow to autophagosome formation.
Title: Post-Golgi Sec proteins are required for autophagy in Saccharomyces cerevisiae.
Mlc1p/Myo2p interaction is stimulated by a signal that requires Sec2p and activation of Ypt32p
Title: GTP drives myosin light chain 1 interaction with the class V myosin Myo2 IQ motifs via a Sec2 RabGEF-mediated pathway.
Sec2 is a highly active exchange factor, in contrast to other proteins previously characterized as Rab exchange factors.
Title: Sec2 is a highly efficient exchange factor for the Rab protein Sec4.
A hypothetical model of the exchange domain of sec2p as a pair of coiled-coil helices that binds to sec4p and facilitates nucleotide disassociation.
Title: Structural insights into the exchange domain of sec2p: expression, purification, crystallization, and preliminary x-ray diffraction data analysis.
The crystal structure of the Sec2p GEF domain at 3.0 A resolution was reported.
Title: Asymmetric coiled-coil structure with Guanine nucleotide exchange activity.
The crystal structure of the Sec2p guanine nucleotide exchange factor domain in a complex with the nucleotide-free Sec4p at 2.7 A resolution, is reported.
Title: Crystal structure of the Sec4p.Sec2p complex in the nucleotide exchanging intermediate state.

Submit: New GeneRIF Correction See all GeneRIFs (11)

Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables guanyl-nucleotide exchange factor activity	IDA Inferred from Direct Assay more info	PubMed
enables guanyl-nucleotide exchange factor activity	IEA Inferred from Electronic Annotation more info
enables phosphatidylinositol-4-phosphate binding	IDA Inferred from Direct Assay more info	PubMed
enables phosphatidylinositol-4-phosphate binding	IMP Inferred from Mutant Phenotype more info	PubMed

Process	Evidence Code	Pubs
involved_in ascospore-type prospore membrane formation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in autophagy	IGI Inferred from Genetic Interaction more info	PubMed
involved_in autophagy	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in exocytosis	IBA Inferred from Biological aspect of Ancestor more info
involved_in exocytosis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein transport	IEA Inferred from Electronic Annotation more info
involved_in sporulation resulting in formation of a cellular spore	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
is_active_in Golgi to plasma membrane transport vesicle	IBA Inferred from Biological aspect of Ancestor more info
is_active_in cell tip	IBA Inferred from Biological aspect of Ancestor more info
located_in cellular bud neck	HDA more info	PubMed
located_in cellular bud neck	IDA Inferred from Direct Assay more info	PubMed
located_in cellular bud neck	IEA Inferred from Electronic Annotation more info
located_in cellular bud tip	IDA Inferred from Direct Assay more info	PubMed
located_in cellular bud tip	IEA Inferred from Electronic Annotation more info
located_in cytoplasmic vesicle	IEA Inferred from Electronic Annotation more info
located_in cytosol	IDA Inferred from Direct Assay more info	PubMed
located_in mating projection tip	HDA more info	PubMed
located_in transport vesicle	IDA Inferred from Direct Assay more info	PubMed
located_in transport vesicle	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: guanine nucleotide exchange factor SEC2

NP_014127.1

Guanyl-nucleotide exchange factor for the small G-protein Sec4p; essential for post-Golgi vesicle transport and for autophagy; associates with the exocyst, via exocyst subunit Sec15p, on secretory vesicles

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001146.8 Reference assembly

Range

126805..129084 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001183110.1 → NP_014127.1 TPA: guanine nucleotide exchange factor SEC2 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_014127.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6W0S2, P17065

UniProtKB/TrEMBL

A6ZRH2, B3LPB1, C7GP91, C8ZFW7

Conserved Domains (3) summary

TIGR02169
Location:4 → 139

SMC_prok_A; chromosome segregation protein SMC, primarily archaeal type

pfam06428
Location:79 → 170

Sec2p; GDP/GTP exchange factor Sec2p

cd21044
Location:216 → 399

Rab11BD_RAB3IP_like; Rab11 binding domain of Rab-3A-interacting protein (RAB3IP), Rab-3A-interacting-like protein 1 (RAB3IL1) and similar proteins