U.S. flag

An official website of the United States government

Format

Send to:

Choose Destination

VPS45 Vps45p [ Saccharomyces cerevisiae S288C ]

Gene ID: 852785, updated on 18-Sep-2024

Summary

Go to the top of the page Help
Official Symbol
VPS45
Official Full Name
Vps45p
Primary source
SGD:S000003063
Locus tag
YGL095C
See related
AllianceGenome:SGD:S000003063; FungiDB:YGL095C; VEuPathDB:YGL095C
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Saccharomyces cerevisiae S288C (strain: S288C)
Lineage
Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as
STT10; VPL28
Summary
Enables SNARE binding activity and unfolded protein binding activity. Involved in several processes, including Golgi vesicle transport; positive regulation of SNARE complex assembly; and vacuole inheritance. Located in Golgi membrane and cytosol. Is extrinsic component of membrane. Part of SNARE complex. Human ortholog(s) of this gene implicated in severe congenital neutropenia 5. Orthologous to human VPS45 (vacuolar protein sorting 45 homolog). [provided by Alliance of Genome Resources, Apr 2022]
NEW
Try the new Gene table
Try the new Transcript table

Genomic context

Go to the top of the page Help
Location:
chromosome: VII
Exon count:
1
Sequence:
Chromosome: VII; NC_001139.9 (328874..330607, complement)

Chromosome VII - NC_001139.9Genomic Context describing neighboring genes Neighboring gene Tos8p Neighboring gene tRNA-Glu Neighboring gene poly(A)-specific ribonuclease Neighboring gene Spc105p

Genomic regions, transcripts, and products

Go to the top of the page Help
Genomic Sequence:
NC_001139.9

Go to nucleotide: Graphics FASTA GenBank

Bibliography

Go to the top of the pageHelp

Related articles in PubMed

  1. Traffic into the prevacuolar/endosomal compartment of Saccharomyces cerevisiae: a VPS45-dependent intracellular route and a VPS45-independent, endocytic route. Bryant NJ, et al. Eur J Cell Biol, 1998 May. PMID 9650782
  2. Mutations in the VPS45 gene, a SEC1 homologue, result in vacuolar protein sorting defects and accumulation of membrane vesicles. Cowles CR, et al. J Cell Sci, 1994 Dec. PMID 7706396
  3. The Thr224Asn mutation in the VPS45 gene is associated with the congenital neutropenia and primary myelofibrosis of infancy. Stepensky P, et al. Blood, 2013 Jun 20. PMID 23599270
  4. The N-terminal peptide of the syntaxin Tlg2p modulates binding of its closed conformation to Vps45p. Furgason ML, et al. Proc Natl Acad Sci U S A, 2009 Aug 25. PMID 19667197, Free PMC Article
  5. Functional homology of mammalian syntaxin 16 and yeast Tlg2p reveals a conserved regulatory mechanism. Struthers MS, et al. J Cell Sci, 2009 Jul 1. PMID 19509055, Free PMC Article

See all (89) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Data show that the SM protein Vps45p, which regulates endosomal trafficking in yeast, binds the conserved N-terminal peptide of the syntaxin Tlg2p.
    Title: The N-terminal peptide of the syntaxin Tlg2p modulates binding of its closed conformation to Vps45p.
  2. Disruption of this high-affinity binding between Vps45p and Tlg2p leads to downregulation of Tlg2p.
    Title: Cellular levels of the syntaxin Tlg2p are regulated by a single mode of binding to Vps45p.
  3. Vps45p binds its cognate SNARE proteins through distinct mechanisms at different stages in the SNARE assembly/disassembly cycle.
    Title: The Sec1p/Munc18 protein Vps45p binds its cognate SNARE proteins via two distinct modes.
Submit: New GeneRIF Correction

Interactions

Go to the top of the page Help
Products Interactant Other Gene Complex Source Pubs Description

General gene information

Go to the top of the page Help

Homology

Gene Ontology Provided by SGD

Function Evidence Code Pubs
enables SNARE binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables unfolded protein binding IMP
Inferred from Mutant Phenotype
more info
 PubMed 
enables unfolded protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
Process Evidence Code Pubs
involved_in Golgi to endosome transport IGI
Inferred from Genetic Interaction
more info
 PubMed 
involved_in Golgi to vacuole transport IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in Golgi vesicle fusion to target membrane IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in cytoplasm to vacuole targeting by the Cvt pathway IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in intracellular protein transport IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in positive regulation of SNARE complex assembly IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in protein targeting to vacuole IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in protein transport IEA
Inferred from Electronic Annotation
more info
  
involved_in vacuolar acidification IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in vacuole inheritance IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in vacuole organization IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in vesicle-mediated transport IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in vesicle-mediated transport IEA
Inferred from Electronic Annotation
more info
  
Component Evidence Code Pubs
is_active_in Golgi membrane IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in Golgi membrane IDA
Inferred from Direct Assay
more info
 PubMed 
part_of SNARE complex IPI
Inferred from Physical Interaction
more info
 PubMed 
located_in cytoplasm IEA
Inferred from Electronic Annotation
more info
  
located_in cytosol IDA
Inferred from Direct Assay
more info
 PubMed 
located_in endomembrane system IEA
Inferred from Electronic Annotation
more info
  
located_in intracellular membrane-bounded organelle IEA
Inferred from Electronic Annotation
more info
  
located_in membrane IEA
Inferred from Electronic Annotation
more info
  
located_in vacuolar membrane IEA
Inferred from Electronic Annotation
more info
  
located_in vacuole IEA
Inferred from Electronic Annotation
more info
  

General protein information

Go to the top of the page Help
Preferred Names
Vps45p
NP_011420.3
  • Protein of the Sec1p/Munc-18 family; essential for vacuolar protein sorting; required for the function of Pep12p and the early endosome/late Golgi SNARE Tlg2p; essential for fusion of Golgi-derived vesicles with the prevacuolar compartment; mutation in human VPS45 is associated with congenital neutropenia and primary myelofibrosis of infancy

NCBI Reference Sequences (RefSeq)

Go to the top of the page Help

NEW Try the new Transcript table

Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_001139.9 Reference assembly

    Range
    328874..330607 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NM_001180960.3 → NP_011420.3  TPA: Vps45p [Saccharomyces cerevisiae S288C]

    See identical proteins and their annotated locations for NP_011420.3

    Status: REVIEWED

    UniProtKB/Swiss-Prot
    D6VU50, P38932
    UniProtKB/TrEMBL
    A6ZU85, B3LHG0, B5VIN1, N1P5F0
    Conserved Domains (1) summary
    COG5158
    Location:3 → 577
    SEC1; Proteins involved in synaptic transmission and general secretion, Sec1 family [Intracellular trafficking and secretion]
Nucleotide Protein
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
P38932.2

Gene LinkOut

The following LinkOut resources are supplied by external providers. These providers are responsible for maintaining the links.

Other Literature Sources
Molecular Biology Databases
Research Materials
Tools
Miscellaneous