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ROK1 RNA-dependent ATPase ROK1 [ Saccharomyces cerevisiae S288C ]

Gene ID: 852704, updated on 18-Sep-2024

Summary

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Official Symbol
ROK1
Official Full Name
RNA-dependent ATPase ROK1
Primary source
SGD:S000003139
Locus tag
YGL171W
See related
AllianceGenome:SGD:S000003139; FungiDB:YGL171W; VEuPathDB:YGL171W
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Saccharomyces cerevisiae S288C (strain: S288C)
Lineage
Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary
Enables RNA helicase activity. Involved in maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) and snoRNA localization. Located in nucleolus. Part of 90S preribosome. Orthologous to human DDX52 (DExD-box helicase 52). [provided by Alliance of Genome Resources, Apr 2022]
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Genomic context

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See ROK1 in Genome Data Viewer
Location:
chromosome: VII
Exon count:
1
Sequence:
Chromosome: VII; NC_001139.9 (182390..184084)

Chromosome VII - NC_001139.9Genomic Context describing neighboring genes Neighboring gene chromatin-binding exonuclease XRN1 Neighboring gene FG-nucleoporin NUP49 Neighboring gene Spo74p Neighboring gene tRNA-Lys

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_001139.9

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. A pre-ribosomal RNA interaction network involving snoRNAs and the Rok1 helicase. Martin R, et al. RNA, 2014 Aug. PMID 24947498, Free PMC Article
  2. Upstream open reading frames regulate the cell cycle-dependent expression of the RNA helicase Rok1 in Saccharomyces cerevisiae. Jeon S, et al. FEBS Lett, 2010 Nov 19. PMID 20969870
  3. ATP hydrolysis activity of the DEAD box protein Rok1p is required for in vivo ROK1 function. Oh JY, et al. Nucleic Acids Res, 1999 Jul 1. PMID 10373593, Free PMC Article
  4. Characterization and intracellular localization of the Rok1 protein involved in yeast cell division. Rhee JY, et al. Mol Cells, 1998 Feb 28. PMID 9571634
  5. Cofactor-dependent specificity of a DEAD-box protein. Young CL, et al. Proc Natl Acad Sci U S A, 2013 Jul 16. PMID 23630256, Free PMC Article

See all (64) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Data show that ATP-bound DEAD-box protein Rok1, but not adenosine diphosphate (ADP)-bound Rok1, stabilizes Rrp5 protein binding to 40S ribosomes.
    Title: The DEAD-box Protein Rok1 Orchestrates 40S and 60S Ribosome Assembly by Promoting the Release of Rrp5 from Pre-40S Ribosomes to Allow for 60S Maturation.
  2. findings revealed that Rok1 protein synthesis is repressed by Psk2 and Skp1; this repression appeared to be mediated through the ROK1 uORF1; Tub4 plays a positive role in regulating Rok1 protein synthesis and likely after the uORF1-mediated inhibitory regulation
    Title: Identification of Psk2, Skp1, and Tub4 as trans-acting factors for uORF-containing ROK1 mRNA in Saccharomyces cerevisiae.
  3. Rrp5 provides specificity to the otherwise nonspecific biochemical activities of the Rok1 DEAD-domain.
    Title: Cofactor-dependent specificity of a DEAD-box protein.
  4. Rok1 was required specifically for the release of snR30.
    Title: Quantitative analysis of snoRNA association with pre-ribosomes and release of snR30 by Rok1 helicase.
  5. Immunoprecipitation assays demonstrate the association of Rok1p with 35S pre-rRNA in pre-ribosomes.
    Title: U3 snoRNP and Rrp5p associate independently with Saccharomyces cerevisiae 35S pre-rRNA, but Rrp5p is essential for association of Rok1p.
Submit: New GeneRIF Correction

Pathways from PubChem

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Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Homology

Gene Ontology Provided by SGD

Function Evidence Code Pubs
enables ATP binding IEA
Inferred from Electronic Annotation
more info
  
enables ATP hydrolysis activity IEA
Inferred from Electronic Annotation
more info
  
enables ATP-dependent activity, acting on RNA IDA
Inferred from Direct Assay
more info
 PubMed 
enables RNA binding IEA
Inferred from Electronic Annotation
more info
  
enables RNA helicase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables RNA helicase activity IEA
Inferred from Electronic Annotation
more info
  
enables helicase activity IEA
Inferred from Electronic Annotation
more info
  
enables hydrolase activity IEA
Inferred from Electronic Annotation
more info
  
enables nucleic acid binding IEA
Inferred from Electronic Annotation
more info
  
Process Evidence Code Pubs
involved_in endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA) IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in maturation of SSU-rRNA IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in maturation of SSU-rRNA IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in maturation of SSU-rRNA IEA
Inferred from Electronic Annotation
more info
  
involved_in rRNA processing IEA
Inferred from Electronic Annotation
more info
  
involved_in ribosome biogenesis IEA
Inferred from Electronic Annotation
more info
  
involved_in snoRNA localization IMP
Inferred from Mutant Phenotype
more info
 PubMed 
Component Evidence Code Pubs
part_of 90S preribosome HDA PubMed 
located_in nucleolus IDA
Inferred from Direct Assay
more info
 PubMed 
located_in nucleolus IEA
Inferred from Electronic Annotation
more info
  
located_in nucleus IEA
Inferred from Electronic Annotation
more info
  

General protein information

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Preferred Names
RNA-dependent ATPase ROK1
NP_011344.1
  • RNA-dependent ATPase; involved in pre-rRNA processing at sites A0, A1, and A2, and in control of cell cycle progression; contains two upstream open reading frames (uORFs) in 5' untranslated region which regulate translation

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_001139.9 Reference assembly

    Range
    182390..184084
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NM_001181036.1 → NP_011344.1  TPA: RNA-dependent ATPase ROK1 [Saccharomyces cerevisiae S288C]

    See identical proteins and their annotated locations for NP_011344.1

    Status: REVIEWED

    UniProtKB/Swiss-Prot
    A6ZU15, D6VTY1, P45818
    UniProtKB/TrEMBL
    B3LHM3, C7GPN6, C8Z880, N1P4C1
    Conserved Domains (2) summary
    COG0513
    Location:101 → 560
    SrmB; Superfamily II DNA and RNA helicase [Replication, recombination and repair]
    cd17957
    Location:133 → 336
    DEADc_DDX52; DEAD-box helicase domain of DEAD box protein 52
Nucleotide Protein
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
P45818.1

Gene LinkOut

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