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PCA1 cation-transporting P-type ATPase PCA1 [ Saccharomyces cerevisiae S288C ]

Gene ID: 852598, updated on 18-Sep-2024

Summary

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Official Symbol
PCA1
Official Full Name
cation-transporting P-type ATPase PCA1
Primary source
SGD:S000000499
Locus tag
YBR295W
See related
AllianceGenome:SGD:S000000499; FungiDB:YBR295W; VEuPathDB:YBR295W
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Saccharomyces cerevisiae S288C (strain: S288C)
Lineage
Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as
CAD2; PAY2
Summary
Enables P-type cadmium transporter activity; copper ion binding activity; and proteasome binding activity. Involved in cadmium ion transport; cellular metal ion homeostasis; and transmembrane transport. Located in plasma membrane. Is integral component of plasma membrane. Part of proteasome core complex. [provided by Alliance of Genome Resources, Apr 2022]
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Genomic context

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See PCA1 in Genome Data Viewer
Location:
chromosome: II
Exon count:
1
Sequence:
Chromosome: II; NC_001134.8 (792849..796499)

Chromosome II - NC_001134.8Genomic Context describing neighboring genes Neighboring gene Vba2p Neighboring gene sulfate permease Neighboring gene Pho89p Neighboring gene Tyc1p

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_001134.8

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. Variation of the response to metal ions and nonsense-mediated mRNA decay across different Saccharomyces cerevisiae genetic backgrounds. Wong A, et al. Yeast, 2021 Sep. PMID 33955055, Free PMC Article
  2. A tradeoff drives the evolution of reduced metal resistance in natural populations of yeast. Chang SL, et al. PLoS Genet, 2011 Mar. PMID 21483812, Free PMC Article
  3. Expressional control of a cadmium-transporting P1B-type ATPase by a metal sensing degradation signal. Adle DJ, et al. J Biol Chem, 2008 Nov 14. PMID 18753133, Free PMC Article
  4. A cadmium-transporting P1B-type ATPase in yeast Saccharomyces cerevisiae. Adle DJ, et al. J Biol Chem, 2007 Jan 12. PMID 17107946, Free PMC Article
  5. The cadmium-resistant gene, CAD2, which is a mutated putative copper-transporter gene (PCA1), controls the intracellular cadmium-level in the yeast S. cerevisiae. Shiraishi E, et al. Curr Genet, 2000 Feb. PMID 10743563

See all (34) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Determined the roles for the molecular factors of ERAD in dislodging Pca1p from the endoplasmic reticulum (ER). Inactivation of the 20S proteasome leads to accumulation of ubiquitinated Pca1p in the ER membrane, suggesting a role for the proteasome in extraction of Pca1p from the ER.
    Title: Endoplasmic Reticulum-associated Degradation of Pca1p, a Polytopic Protein, via Interaction with the Proteasome at the Membrane.
  2. Data indicate that specific cysteine residues in a degron of cation-transporting P-type ATPase PCA1 (Pca1p) sense cadmium.
    Title: Cadmium and Secondary Structure-dependent Function of a Degron in the Pca1p Cadmium Exporter.
  3. cadmium resistance is primarily caused by an enhanced function of a metal efflux pump, PCA1.
    Title: A tradeoff drives the evolution of reduced metal resistance in natural populations of yeast.
  4. Direct cadmium sensing at the endoplasmic reticulum by a degron in Pca1 leads to an escape of Pca1 from endoplasmic reticulum-associated degradation.
    Title: Cadmium-mediated rescue from ER-associated degradation induces expression of its exporter.
  5. Cadmium sensing by cysteine residues within this domain circumvents ubiquitination and degradation of Pca1
    Title: Expressional control of a cadmium-transporting P1B-type ATPase by a metal sensing degradation signal.
  6. Overexpression or deletion of the functional allele in yeast demonstrates that PCA1 is a cadmium efflux pump
    Title: A cadmium-transporting P1B-type ATPase in yeast Saccharomyces cerevisiae.
Submit: New GeneRIF Correction

Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Homology

Gene Ontology Provided by SGD

Function Evidence Code Pubs
enables ATP binding IEA
Inferred from Electronic Annotation
more info
  
enables ATP hydrolysis activity IEA
Inferred from Electronic Annotation
more info
  
enables ATPase-coupled monoatomic cation transmembrane transporter activity IEA
Inferred from Electronic Annotation
more info
  
enables P-type cadmium transporter activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables P-type cadmium transporter activity IEA
Inferred from Electronic Annotation
more info
  
enables P-type cadmium transporter activity IMP
Inferred from Mutant Phenotype
more info
 PubMed 
enables copper ion binding IBA
Inferred from Biological aspect of Ancestor
more info
  
enables copper ion binding IMP
Inferred from Mutant Phenotype
more info
 PubMed 
enables metal ion binding IEA
Inferred from Electronic Annotation
more info
  
enables proteasome binding IPI
Inferred from Physical Interaction
more info
 PubMed 
Process Evidence Code Pubs
involved_in cadmium ion transmembrane transport IEA
Inferred from Electronic Annotation
more info
  
involved_in cadmium ion transport IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in detoxification of copper ion IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in intracellular monoatomic cation homeostasis IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in monoatomic cation transmembrane transport IEA
Inferred from Electronic Annotation
more info
  
involved_in monoatomic cation transport IEA
Inferred from Electronic Annotation
more info
  
involved_in monoatomic ion transport IEA
Inferred from Electronic Annotation
more info
  
involved_in response to cadmium ion IEA
Inferred from Electronic Annotation
more info
  
involved_in transmembrane transport IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in transmembrane transport IMP
Inferred from Mutant Phenotype
more info
 PubMed 
Component Evidence Code Pubs
located_in membrane IEA
Inferred from Electronic Annotation
more info
  
is_active_in plasma membrane IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in plasma membrane IDA
Inferred from Direct Assay
more info
 PubMed 
located_in plasma membrane IEA
Inferred from Electronic Annotation
more info
  
part_of proteasome core complex IDA
Inferred from Direct Assay
more info
 PubMed 

General protein information

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Preferred Names
cation-transporting P-type ATPase PCA1
NP_009854.2
  • Cadmium transporting P-type ATPase; may also have a role in copper and iron homeostasis; stabilized by Cd binding, which prevents ubiquitination; S288C and other lab strains contain a G970R mutation which eliminates Cd transport function

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_001134.8 Reference assembly

    Range
    792849..796499
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NM_001178643.2 → NP_009854.2  TPA: cation-transporting P-type ATPase PCA1 [Saccharomyces cerevisiae S288C]

    See identical proteins and their annotated locations for NP_009854.2

    Status: REVIEWED

    UniProtKB/Swiss-Prot
    D6VQT9, P38360
    UniProtKB/TrEMBL
    A6ZLN2, B3LML9, C7GMT8, N1PAW9
    Conserved Domains (2) summary
    cd00371
    Location:413 → 471
    HMA; Heavy-metal-associated domain (HMA) is a conserved domain of approximately 30 amino acid residues found in a number of proteins that transport or detoxify heavy metals, for example, the CPx-type heavy metal ATPases and copper chaperones. HMA domain ...
    TIGR01511
    Location:605 → 1193
    ATPase-IB1_Cu; copper-(or silver)-translocating P-type ATPase
Nucleotide Protein
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
P38360.2

Gene LinkOut

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