RPT5A regulatory particle triple-A ATPase 5A [Arabidopsis thaliana (thale cress)] - Gene

Summary

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Gene symbol: RPT5A
Gene description: regulatory particle triple-A ATPase 5A
Primary source: Araport:AT3G05530
Locus tag: AT3G05530
See related: TAIR:AT3G05530
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Arabidopsis thaliana (ecotype: Columbia)
Lineage: Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
Also known as: 19S PROTEASOME REGULATORY COMPLEX SUBUNIT S6A; 26S PROTEASOME AAA-ATPASE SUBUNIT; ATS6A.2; F22F7.1; F22F7_1; regulatory particle triple-A ATPase 5A
Summary: Encodes RPT5a (Regulatory Particle 5a), one of the six AAA-ATPases of the proteasome regulatory particle. Essential for gametophyte development. In Arabidopsis, the RPT5 subunit is encoded by two highly homologous genes, RPT5a and RPT5b. RPT5a and RPT5b show accession-dependent functional redundancy. In Wassilewskija (Ws) accession: mutant alleles of RPT5a displayed 50% pollen lethality, indicating that RPT5a is essential for male gametophyte development. In the Columbia (Col) accession, a rpt5a mutant allele did not display such a phenotype because the RPT5b Col allele complements the rpt5a defect in the male gametophyte, whereas the RPT5b Ws allele does not. Double rpt5a rpt5b mutants in Col background showed a complete male and female gametophyte lethal phenotype.
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Genomic context

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See RPT5A in Genome Data Viewer

Location:: chromosome: 3

Exon count:: 10

Sequence:: Chromosome: 3; NC_003074.8 (1603359..1606243)

Chromosome 3 - NC_003074.8 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_003074.8

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

These results suggest that the ahg12 mutation, a missense mutation in RPT5a, led to changes in the substrate preference of the 26S proteasome.
Title: ahg12 is a dominant proteasome mutant that affects multiple regulatory systems for germination of Arabidopsis.
RPT2a and RPT5a are involved in Zn deficiency-tolerance, possibly through alleviation of oxidative stresses and/or processing of poly-ubiquitinated proteins.
Title: Arabidopsis thaliana 26S proteasome subunits RPT2a and RPT5a are crucial for zinc deficiency-tolerance.
The accession-dependent unequal redundancy between RPT5a and RPT5b genes illustrates an example of evolutionary drifting between duplicated genes through alternative splicing
Title: A SNP associated with alternative splicing of RPT5b causes unequal redundancy between RPT5a and RPT5b among Arabidopsis thaliana natural variation.
RPT5 subunits are essential for both male and female gametophyte development.
Title: The Arabidopsis proteasome RPT5 subunits are essential for gametophyte development and show accession-dependent redundancy.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

NM_111426.4

Supported by ESTs:

Gene Ontology Provided by TAIR

Function	Evidence Code	Pubs
enables ATP hydrolysis activity	IEA Inferred from Electronic Annotation more info
enables ATP hydrolysis activity	IGI Inferred from Genetic Interaction more info	PubMed
enables calmodulin binding	TAS Traceable Author Statement more info	PubMed

Process	Evidence Code	Pubs
acts_upstream_of_or_within embryo sac development	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within pollen development	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within proteasomal protein catabolic process	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within ubiquitin-dependent protein catabolic process	TAS Traceable Author Statement more info	PubMed

Component	Evidence Code	Pubs
located_in cytoplasm	HDA more info	PubMed
located_in cytosol	HDA more info	PubMed
located_in nucleus	HDA more info	PubMed
located_in nucleus	ISM Inferred from Sequence Model more info
located_in nucleus	TAS Traceable Author Statement more info	PubMed
part_of proteasome complex	IDA Inferred from Direct Assay more info	PubMed
part_of proteasome regulatory particle, base subcomplex	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: regulatory particle triple-A ATPase 5A

NP_187204.1

regulatory particle triple-A ATPase 5A (RPT5A); CONTAINS InterPro DOMAIN/s: ATPase, AAA+ type, core (InterPro:IPR003593), ATPase, AAA-type, core (InterPro:IPR003959), ATPase, AAA-type, conserved site (InterPro:IPR003960), 26S proteasome subunit P45 (InterPro:IPR005937); BEST Arabidopsis thaliana protein match is: 26S proteasome AAA-ATPase subunit RPT5B (TAIR:AT1G09100.1); Has 33265 Blast hits to 30927 proteins in 3145 species: Archae - 1437; Bacteria - 12044; Metazoa - 4947; Fungi - 3672; Plants - 3322; Viruses - 56; Other Eukaryotes - 7787 (source: NCBI BLink).

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_003074.8 Reference assembly

Range

1603359..1606243

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_111426.4 → NP_187204.1 regulatory particle triple-A ATPase 5A [Arabidopsis thaliana]

See identical proteins and their annotated locations for NP_187204.1

Status: REVIEWED

UniProtKB/Swiss-Prot

Q8H195, Q9SEI2, Q9SWY7

UniProtKB/TrEMBL

A0A178VEP3, A0A5S9XA09, A0A654F9N5

Conserved Domains (1) summary

COG1222
Location:29 → 417

RPT1; ATP-dependent 26S proteasome regulatory subunit [Posttranslational modification, protein turnover, chaperones]