U.S. flag

An official website of the United States government

Format

Send to:

Choose Destination

raf1 CLRC ubiquitin ligase complex WD repeat subunit Raf1/Dos1 [ Schizosaccharomyces pombe (fission yeast) ]

Gene ID: 2538848, updated on 18-Sep-2024

Summary

Go to the top of the page Help
Official Symbol
raf1
Official Full Name
CLRC ubiquitin ligase complex WD repeat subunit Raf1/Dos1
Primary source
PomBase:SPCC613.12c
Locus tag
SPOM_SPCC613.12C
Gene type
protein coding
RefSeq status
PROVISIONAL
Organism
Schizosaccharomyces pombe (strain: 972h-)
Lineage
Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina; Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae; Schizosaccharomyces
Also known as
clr8; cmc1; dos1
NEW
Try the new Gene table
Try the new Transcript table

Genomic context

Go to the top of the page Help
Location:
chromosome: III
Exon count:
1
Sequence:
Chromosome: III; NC_003421.2 (99716..102264, complement)

Chromosome III - NC_003421.2Genomic Context describing neighboring genes Neighboring gene ncRNA Neighboring gene tRNA-splicing endonuclease subunit Sen54 Neighboring gene ncRNA Neighboring gene ubiquinol-cytochrome-c reductase complex core protein Qcr2 Neighboring gene protein meu23 Neighboring gene ncRNA Neighboring gene ATP-dependent helicase Neighboring gene ncRNA Neighboring gene uncharacterized protein Neighboring gene DNA repair protein

Genomic regions, transcripts, and products

Go to the top of the page Help
Genomic Sequence:
NC_003421.2

Go to nucleotide: Graphics FASTA GenBank

Bibliography

Go to the top of the pageHelp

Related articles in PubMed

  1. Lid2 is required for coordinating H3K4 and H3K9 methylation of heterochromatin and euchromatin. Li F, et al. Cell, 2008 Oct 17. PMID 18957202, Free PMC Article
  2. Cell cycle-dependent deposition of CENP-A requires the Dos1/2-Cdc20 complex. Gonzalez M, et al. Proc Natl Acad Sci U S A, 2013 Jan 8. PMID 23267073, Free PMC Article
  3. The Clr7 and Clr8 directionality factors and the Pcu4 cullin mediate heterochromatin formation in the fission yeast Schizosaccharomyces pombe. Thon G, et al. Genetics, 2005 Dec. PMID 16157682, Free PMC Article
  4. Two novel proteins, dos1 and dos2, interact with rik1 to regulate heterochromatic RNA interference and histone modification. Li F, et al. Curr Biol, 2005 Aug 23. PMID 16040243
  5. CRL4-like Clr4 complex in Schizosaccharomyces pombe depends on an exposed surface of Dos1 for heterochromatin silencing. Kuscu C, et al. Proc Natl Acad Sci U S A, 2014 Feb 4. PMID 24449894, Free PMC Article

See all (25) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Disruption of Dos1, Dos2, or Cdc20 results in dissociation of CENP-A from centromeres and mislocalization of the protein to noncentromeric sites during S phase.
    Title: Cell cycle-dependent deposition of CENP-A requires the Dos1/2-Cdc20 complex.
  2. Thus the association of a DCAF, Raf1, with its adaptor, Rik1, is required for histone methylation and to allow RNAi to signal to chromatin
    Title: Raf1 Is a DCAF for the Rik1 DDB1-like protein and has separable roles in siRNA generation and chromatin modification.
  3. Dos1 acts in the RNAi-dependent heterochromatin pathway. It is required for silencing of heterochromatin regions, for H3K9me association with centromeric repeats, for production of siRNAs, and for Swi6 localization. It directly interacts with Rik1. [dos1]
    Title: Two novel proteins, dos1 and dos2, interact with rik1 to regulate heterochromatic RNA interference and histone modification.
Submit: New GeneRIF Correction

Interactions

Go to the top of the page Help
Products Interactant Other Gene Complex Source Pubs Description

General gene information

Go to the top of the page Help

Homology

Gene Ontology Provided by PomBase

Function Evidence Code Pubs
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
Process Evidence Code Pubs
involved_in CENP-A containing chromatin assembly IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in meiotic telomere clustering IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in siRNA-mediated pericentric heterochromatin formation IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in silent mating-type cassette heterochromatin formation IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in subtelomeric heterochromatin formation IMP
Inferred from Mutant Phenotype
more info
 PubMed 
Component Evidence Code Pubs
part_of CLRC complex IDA
Inferred from Direct Assay
more info
 PubMed 
is_active_in chromatin IDA
Inferred from Direct Assay
more info
 PubMed 
is_active_in cytosol HDA PubMed 
is_active_in mating-type region heterochromatin IDA
Inferred from Direct Assay
more info
 PubMed 
is_active_in nucleus HDA PubMed 
is_active_in nucleus IBA
Inferred from Biological aspect of Ancestor
more info
  
is_active_in nucleus IDA
Inferred from Direct Assay
more info
 PubMed 
is_active_in pericentric heterochromatin IDA
Inferred from Direct Assay
more info
 PubMed 
is_active_in subtelomeric heterochromatin IDA
Inferred from Direct Assay
more info
 PubMed 

General protein information

Go to the top of the page Help
Preferred Names
CLRC ubiquitin ligase complex WD repeat subunit Raf1/Dos1

NCBI Reference Sequences (RefSeq)

Go to the top of the page Help

NEW Try the new Transcript table

Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_003421.2 Reference assembly

    Range
    99716..102264 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NM_001022695.3 → NP_587700.1  CLRC ubiquitin ligase complex WD repeat subunit Raf1/Dos1 [Schizosaccharomyces pombe]

    See identical proteins and their annotated locations for NP_587700.1

    Status: PROVISIONAL

    UniProtKB/Swiss-Prot
    O74910
    Conserved Domains (3) summary
    COG2319
    Location:300 → 622
    WD40; WD40 repeat [General function prediction only]
    sd00039
    Location:302 → 338
    7WD40; WD40 repeat [structural motif]
    cl02567
    Location:302 → 607
    WD40; WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions including adaptor/regulatory modules in signal transduction, pre-mRNA processing and cytoskeleton assembly; typically contains a GH dipeptide 11-24 residues from ...
Nucleotide Protein
Heading Accession and Version

Gene LinkOut

The following LinkOut resources are supplied by external providers. These providers are responsible for maintaining the links.