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Human cultured dermal papilla cell cDNA library(M)

Identifiers
BioSample: SAMN00152855; EST: LIBEST_021100
Organism
Homo sapiens (human)
cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Sarcopterygii; Dipnotetrapodomorpha; Tetrapoda; Amniota; Mammalia; Theria; Eutheria; Boreoeutheria; Euarchontoglires; Primates; Haplorrhini; Simiiformes; Catarrhini; Hominoidea; Hominidae; Homininae; Homo
Attributes
tissuebeard hair
cell typedermal papilla
development stagePrimary culture
lab hostSOLR
tissueskin
vectorpBluescript SK-
v_typeplasmid
re_1EcoRI
re_2XhoI
Description

Library was constructed using ZAP-cDNA synthesis kit (Stratagene). mRNA was prepared from cultured dermal papilla cells which isolated from beard. Cloned unidirectionally. Primer: Oligo dT. Average insert size: 1.5 kb. Uni-ZAP XR vector: 5' adaptor sequence, 5'-GAATTCGGCACGAG-3'; 3' adaptor sequence, 5'-CTCGAGTTTTTTTTTTTTTTTTTT-3'. Size fractionated to remove sequence <500 bp in size. Amplified once.

Submission
Kyungpook National University School of Medicine, Kim JC; 2007-04-12
Accession:
SAMN00152855
ID:
152855

Nucleotide

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