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Tao Tao, PhD


The National Center for Biotechnology Information has been providing a free sequence alignment service to the public using the BLAST family of programs for the past 15 years. Currently the service is provided through the NCBI BLAST web server located at Multiple venues are available for end users to submit search requests to this service. This document provides a general introduction to the NCBI BLAST server and explains how the BLAST search requests are handled. The focus is on describing the URL encoded parameters and their accepted values in detail. Additional information on key parameters and their values, usage guideline, sample URLs for different searches, and example perl scripts is also provided. This updated document includes the parameters introduced to the new BLAST pages released on April 17, 2007.

Tao Tao 2007-08-03