show Abstracthide AbstractMice (C57Bl/6J) that are fed increasing levels of dietary fat (0, 10, and 60 Kcal%) show a parallel increasing decline in global promoter-H3K9-butyryl (H3K9Bu), but none in promoter-H3K9-acetyl (H3K9ac), in the heart. Additionally, transverse aortic constriction (TAC) induces a further decline in H3K9Bu, compared to an increase in H3K9ac. Overall design: C57Bl/6J mice were fed either a fat-free (0 Kcal%, Fat 0), low-fat (10 Kcal%, Fat 10 or control), or high-fat (60 Kcal%, Fat 60 or HiFat) diet for 4 days before subjecting them to a sham or transverse aortic constriction (TAC) surgeries. The mice were maintained on the same diet for another week, before they were assessed by echocardiography, the hearts isolated, chromatin extracted , and subjected to anti-H3K9Bu and anti-H3K9ac ChIP-Seq