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SRX9898622: GSM5026614: cDC1 WT replicate 3; Mus musculus; RNA-Seq
1 ILLUMINA (NextSeq 500) run: 31.1M spots, 2.7G bases, 1.2Gb downloads

Submitted by: NCBI (GEO)
Study: RNA-seq analysis of mouse cDC1 and cDC2 populations upon DC-SCRIPT depletion
show Abstracthide Abstract
The functional diversification of dendritic cells (DCs) is a key step in establishing protective immune responses. Despite the importance of this lineage diversity, its genetic basis is not fully understood. DC-SCRIPT (Zfp366) is a poorly known transcription factor expressed in conventional DCs (cDCs) and their committed bone marrow progenitors but not in plasmacytoid DCs (pDCs). We show that mice lacking DC-SCRIPT displayed substantially impaired development of IRF8-dependent conventional DC1 (cDC1), while cDC2 differentiated normally. The residual DC-SCRIPT-deficient cDC1s had impaired CD8+ T-cell cross-priming, which could be in part explained by the direct control of DC-SCRIPT on IL-12p40 production. Genome-wide mapping of DC-SCRIPT binding and gene expression analyses revealed a key role for DC-SCRIPT in maintaining cDC1 identity via the direct regulation of cDC1 signature genes, including Irf8. Our study reveals DC-SCRIPT to be a critical component of the gene regulatory program shaping the functional attributes of cDC1s. Overall design: cDC1s and cDC2s were sorted from the spleens of WT and Zfp366-/- mice. RNA was extracted and profiled using RNA-seq. Biological triplicates were prepared for each genotype to produce twelve samples in total.
Sample: cDC1 WT replicate 3
SAMN17384736 • SRS8077662 • All experiments • All runs
Organism: Mus musculus
Library:
Instrument: NextSeq 500
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: SINGLE
Construction protocol: RNeasy Plus Mini kit (Qiagen). TruSeq RNA Sample preparation kit (Illumina)
Experiment attributes:
GEO Accession: GSM5026614
Links:
Runs: 1 run, 31.1M spots, 2.7G bases, 1.2Gb
Run# of Spots# of BasesSizePublished
SRR1348601631,091,5052.7G1.2Gb2021-02-25

ID:
12951471

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