GSM4962794: inflamed gut sample S40 from patient M; human gut metagen... - SRA

SRX9649159: GSM4962794: inflamed gut sample S40 from patient M; human gut metagenome; OTHER
1 ILLUMINA (Illumina MiSeq) run: 144,645 spots, 76.9M bases, 43.3Mb downloads

Submitted by: NCBI (GEO)

Study: 16S V3-V4 -based microbial profiling of matched healthy and inflamed ileal mucosa from patients affected by Crohn's disease

PRJNA683584 • SRP297049 • All experiments • All runs

show Abstracthide Abstract

Crohn's Disease (CD) pathogenesis is still unclear. Disorders in the mucosal immunoregulation and its crosstalk with the microbiota may represent an important component in tissue injury. We aimed to characterize the molecular immune response distribution within the ileal layers ( mucosa, submucosa and serosa) and to evaluate the correlated microbiota in pathological/healthy settings comparing first surgery/relapse clinical conditions. Overall design: 10 matched pairs of paraffin-embedded ileal tissue specimens was extracted and 16S rDNA amplicons (V3-V4 region)

Sample: inflamed gut sample S40 from patient M

SAMN17025378 • SRS7848508 • All experiments • All runs

Organism: human gut metagenome

Library:

Instrument: Illumina MiSeq

Strategy: OTHER

Source: GENOMIC

Selection: other

Layout: PAIRED

Construction protocol: deparaffinatin with xylene followed by DNA extration with QIAamp DNA FFPE tissue kit (QIAGEN) Illumina 16S Metagenomic Sequencing Library Preparation protocol ( see https://emea.support.illumina.com)

Experiment attributes:

GEO Accession: GSM4962794

Links:

NCBI link: NCBI Entrez (gds)

Runs: 1 run, 144,645 spots, 76.9M bases, 43.3Mb

Run	# of Spots	# of Bases	Size	Published
SRR13216208	144,645	76.9M	43.3Mb	2021-11-23

ID:: 12613690

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