GSM4962786: inflamed gut sample S30 from patient M; human gut metagen... - SRA

SRX9649151: GSM4962786: inflamed gut sample S30 from patient M; human gut metagenome; OTHER
1 ILLUMINA (Illumina MiSeq) run: 103,991 spots, 57.8M bases, 32.7Mb downloads

Submitted by: NCBI (GEO)

Study: 16S V3-V4 -based microbial profiling of matched healthy and inflamed ileal mucosa from patients affected by Crohn's disease

PRJNA683584 • SRP297049 • All experiments • All runs

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Crohn's Disease (CD) pathogenesis is still unclear. Disorders in the mucosal immunoregulation and its crosstalk with the microbiota may represent an important component in tissue injury. We aimed to characterize the molecular immune response distribution within the ileal layers ( mucosa, submucosa and serosa) and to evaluate the correlated microbiota in pathological/healthy settings comparing first surgery/relapse clinical conditions. Overall design: 10 matched pairs of paraffin-embedded ileal tissue specimens was extracted and 16S rDNA amplicons (V3-V4 region)

Sample: inflamed gut sample S30 from patient M

SAMN17025371 • SRS7848500 • All experiments • All runs

Organism: human gut metagenome

Library:

Instrument: Illumina MiSeq

Strategy: OTHER

Source: GENOMIC

Selection: other

Layout: PAIRED

Construction protocol: deparaffinatin with xylene followed by DNA extration with QIAamp DNA FFPE tissue kit (QIAGEN) Illumina 16S Metagenomic Sequencing Library Preparation protocol ( see https://emea.support.illumina.com)

Experiment attributes:

GEO Accession: GSM4962786

Links:

NCBI link: NCBI Entrez (gds)

Runs: 1 run, 103,991 spots, 57.8M bases, 32.7Mb

Run	# of Spots	# of Bases	Size	Published
SRR13216200	103,991	57.8M	32.7Mb	2021-11-23

ID:: 12613682

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