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SRX9633984: GSM4957616: input DNA for PS10; Mus musculus; ChIP-Seq
2 ILLUMINA (Illumina HiSeq 2500) runs: 36.4M spots, 1.8G bases, 612.7Mb downloads

Submitted by: NCBI (GEO)
Study: Genome-wide maps of transcription factor binding and histone modifications in splenic resting B cells isolated from mice
show Abstracthide Abstract
Runx1 in resting B cells co-localises with the nucleosome remodeler subunit SRCAP and with the polycomb factor RING1B at promoter and enhancers of active and poised genes. Overall design: Resting B cells were isolated from spleen of wild-type mice by using an EasySep Negative Selection- Mouse B Cell Isolation Kit (STEMCELL Technologies). The cells from replicates designated PS1,2,3,5,10,11,12 were formaldehyde-fixed and ChIP was carried out.
Sample: input DNA for PS10
SAMN17004673 • SRS7834109 • All experiments • All runs
Organism: Mus musculus
Library:
Instrument: Illumina HiSeq 2500
Strategy: ChIP-Seq
Source: GENOMIC
Selection: ChIP
Layout: SINGLE
Construction protocol: Spleens were isolated from 6- to 10-week-old C57B6 mice and homogenized through a sieve in B cell culture medium (RPMI-1640 (Lonza), 10% fetal calf serum (FCS) (Sigma), 0.1 U/ml penicillin (Lonza), 0.1 μg/ml streptomycin (Lonza), 2mM L-Glutamine (Lonza), 50μM beta-mercaptoethanol (Gibco)). The cell suspension was centrifuged on a Ficoll-Paque (GE Healthcare) cushion and the buffy coat layer was resuspended at a concentration of 1x 10e8 cells/ml in PBS + 2% FCS/ 1mM EDTA. Resting B cells were isolated using an EasySep Negative Selection- Mouse B Cell Isolation Kit (STEMCELL Technologies), which depletes for non-B cell markers and activated CD43+ B cells. 5ng of ChIP/Input DNA was used to prepare libraries using the NEBNext® Ultra™ II DNA LibraryPrep Kit for Illumina following the manufacturer recommendations. Library quality and quantity were assessed on a Bioanalyser and Qubit, respectively. Libraries were sequenced on an Illumina Hiseq 2500 (v4 chemistry), and single-end 50bp reads or paired-end 100bp reads per sample were generated.
Experiment attributes:
GEO Accession: GSM4957616
Links:
Runs: 2 runs, 36.4M spots, 1.8G bases, 612.7Mb
Run# of Spots# of BasesSizePublished
SRR1320048118,359,985918M310.3Mb2021-10-01
SRR1320048218,056,117902.8M302.4Mb2021-10-01

ID:
12593612

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