Instrument: Illumina HiSeq 2500
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: SINGLE
Construction protocol: CD34 positive cord blood cells were isolated using magnetic beads from fresh cord blood (For each biological replicate, three different cord blood samples were mixed). The cell were retrovirally infected with ETV6-NCOA2 or empty vector and cultured in IMDM supplemented with hSCF, hTPO, hFlt3L (100 ng/ml) for 5 days. The positive cells were sorted (GFP). ETV6-NCOA2 or ETV6-NCOA2+NOTCH1-L1601PdP were sorted (NGFR+/ GFP+NGFR+ respectively) from fresh bone marrow of engrafted NSGS mice. ETV6-NCOA2 patient derived xenografts were sorted with human CD45 from bone marrow of engrafted NSGS mice. We extracted RNA from the samples and performed bulk RNA-sequencing. Inhouse poly A based RNA seq