GSM4905630: O_W0D1_O1_Unprotected; Macaca mulatta; RNA-Seq - SRA

SRX9498850: GSM4905630: O_W0D1_O1_Unprotected; Macaca mulatta; RNA-Seq
1 ILLUMINA (NextSeq 500) run: 25.5M spots, 3.9G bases, 1.4Gb downloads

Submitted by: NCBI (GEO)

Study: Host response to RhCMV/SIV immunization through subcutaneous and oral route

PRJNA677978 • SRP292387 • All experiments • All runs

show Abstracthide Abstract

Strain 68-1–derived Rhesus Cytomegalovirus (RhCMV) vectors expressing simian immunodeficiency virus (SIV) proteins (RhCMV/SIV) are able to elicit and maintain cellular immune responses that stringently control and subsequently clear a mucosal challenge with highly pathogenic SIV in 50-60% of vaccinated rhesus monkeys (RMs). Here, we utilize whole blood transcriptomic profiling to identify host responses correlated to RhCMV/SIV efficacy. Overall design: Longitudinal mRNA profiling of whole blood from rhesus macaques during immunization with RhCMV/SIV.

Sample: O_W0D1_O1_Unprotected

SAMN16784723 • SRS7707254 • All experiments • All runs

Organism: Macaca mulatta

Library:

Instrument: NextSeq 500

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: PAIRED

Construction protocol: Whole blood was collected from RM in PAXgene RNA tubes (PreAnalytiX) following the manufacturer's procedures. (PreAnalytiX). RNA was isolated using PAXgene Blood miRNA kits (Qiagen) following the protocol provided with the kit that included an on-column DNase treatment. Illumina TruSeq® Stranded mRNA HT kit

Experiment attributes:

GEO Accession: GSM4905630

Links:

NCBI link: NCBI Entrez (gds)

Runs: 1 run, 25.5M spots, 3.9G bases, 1.4Gb

Run	# of Spots	# of Bases	Size	Published
SRR13049496	25,516,641	3.9G	1.4Gb	2021-06-29

ID:: 12414318

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