GSM4891930: 20E -> JH3 - 30 min - rep 3; Harpegnathos saltator; RNA-S... - SRA

SRX9470918: GSM4891930: 20E -> JH3 - 30 min - rep 3; Harpegnathos saltator; RNA-Seq
1 ILLUMINA (NextSeq 550) run: 16.3M spots, 1.2G bases, 446Mb downloads

Submitted by: NCBI (GEO)

Study: RNA-seq in primary Harpegnathos neuronal cultures stimulated with 20E or JH3

PRJNA675869 • SRP292032 • All experiments • All runs

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We cultured primary Harpegnathos neurons in a medium containing 20E or JH3 and then switched the hormones. RNA-seq was performed 30 minutes, 6 hours, or 24 hours after the switch. To determine baseline expression in the cultures before the switch we also performed RNA-seq on the 6-day-old cultures 30 minues after addition of vehicle (DMSO). Overall design: At least 3 independent cultures (biological replicates) per condition and time point

Sample: 20E -> JH3 - 30 min - rep 3

SAMN16727709 • SRS7681270 • All experiments • All runs

Organism: Harpegnathos saltator

Library:

Instrument: NextSeq 550

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: PAIRED

Construction protocol: Cells were lysed in TRIzol (Thermo Fisher Scientific, MA). RNA was purified and its quality visualized on agarose-formaldehyde gels. Typical yields amounted to 0.6 µg total RNA per central brain. For library preparation, polyA+ RNA was isolated from 600 ng total RNA using Dynabeads Oligo(dT)25 (Thermo Fisher) and constructed into strand-specific libraries using the dUTP method (Parkhomchuk et al., 2009). UTP-marked cDNA was end-repaired using end-repair mix (Enzymatics, MA), tailed with deoxyadenine using Klenow exo- (Enzymatics), and ligated to custom dual- indexed adapters with T4 DNA ligase (Enzymatics). Libraries were size-selected with SPRIselect beads (Beckman Coulter, CA) and quantified by qPCR after amplification.

Experiment attributes:

GEO Accession: GSM4891930

Links:

NCBI link: NCBI Entrez (gds)

Runs: 1 run, 16.3M spots, 1.2G bases, 446Mb

Run	# of Spots	# of Bases	Size	Published
SRR13020641	16,294,808	1.2G	446Mb	2021-11-05

ID:: 12377877

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