Instrument: Illumina HiSeq 4000
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: PAIRED
Construction protocol: Animals post-natal day 1, 4 weeks, 14 weeks, 1 year and 1.5 years old were used for determination of gene expression analysis. PDGFR⍺ cardiac fibroblasts were isolated by flow cytometry from hearts of animals at the above respective ages to determine changes in gene expression in cardiac fibroblasts. RNA was isolated from the heart tissue or cardiac fibroblasts using RNeasy Mini kit (Qiagen) and used to generate RNA-Seq libraries followed by sequencing using Illumina 4000 platform.