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SRX8820358: GSM4695474: G1E-ER4 24h - chiapet-SMC1A_rep1; Mus musculus; OTHER
1 ILLUMINA (NextSeq 500) run: 299.4M spots, 89.8G bases, 36.2Gb downloads

Submitted by: NCBI (GEO)
Study: GATA Switch Enhancers Mark Dynamically Regulated Chromatin Interaction Nodes during Erythroid Maturation
show Abstracthide Abstract
Erythropoiesis is among the best understood examples of cell differentiation. In a required step, GATA1 replaces GATA2 at genomic loci, termed GATA switch enhancers. Little is known about how this switch affects chromatin architecture. Here, we show that cohesin binds GATA1 and occupies most GATA switch enhancers. Cohesin-based chromatin interaction analysis demonstrated that the most dynamic and interactive enhancers are enriched for GATA switch sites/TAL1 occupancy and frequently lack super-enhancer features. A c-kit locus CRISPR/Cas9 screen identified GATA motifs within one such element as strongly impacting expression. These data highlight the role of developmentally exchanged transcription factors in modulating genomic folding. Overall design: Epigenetic profiling of erythroid differentiation
Sample: G1E-ER4 24h - chiapet-SMC1A_rep1
SAMN15641186 • SRS7083911 • All experiments • All runs
Organism: Mus musculus
Library:
Instrument: NextSeq 500
Strategy: OTHER
Source: GENOMIC
Selection: other
Layout: PAIRED
Construction protocol: ChIA-PET was performed as previously described (Hnisz et al., Science, 2016) using G1-ER4 cells (150 million per replicate)
Experiment attributes:
GEO Accession: GSM4695474
Links:
Runs: 1 run, 299.4M spots, 89.8G bases, 36.2Gb
Run# of Spots# of BasesSizePublished
SRR12319379299,400,50789.8G36.2Gb2020-10-02

ID:
11467264

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