GSM4663610: Trametes versicolor 1956-1252 2w-3; Trametes versicolor; ... - SRA

SRX8698145: GSM4663610: Trametes versicolor 1956-1252 2w-3; Trametes versicolor; RNA-Seq
1 ILLUMINA (Illumina HiSeq 2000) run: 47.7M spots, 9.5G bases, 5Gb downloads

Submitted by: NCBI (GEO)

Study: Gene expression analysis of two strains of Trametes versicolor

PRJNA645074 • SRP271072 • All experiments • All runs

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Illumina HiSeq technology was used to generate mRNA profiles from two strains of Trametes versicolor. Mycelium of Trametes versicolor BRFM1218 and Trametes versicolor 1956-1252 were harvested after 2 and 4 weeks of incubation on 4% malt agar medium and used for total RNA extraction. Paired-end reads of 100 bp were generated and aligned to Trametes versicolor (https://mycocosm.jgi.doe.gov/Trave1/Trave1.home.html) reference transcripts using CLC Genomics Workbench 7.5.1. Overall design: mRNA profiles from from two strains of Trametes versicolor (BRFM1218 and 1956-1252) were generated by Illumina HiSeq2000 sequencing (2x100bp). Three biological replicates were sequenced from mycelium grown for 2 or 4 weeks on 4% malt agar medium.

Sample: Trametes versicolor 1956-1252 2w-3

SAMN15491707 • SRS6976023 • All experiments • All runs

Organism: Trametes versicolor

Library:

Instrument: Illumina HiSeq 2000

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: PAIRED

Construction protocol: Total RNA was extracted and purified from triplicate cultures using the RNeasy Plant Mini Kit cDNA libraries were prepared for sequencing using standard Illumina protocols by Beckman Coulter Genomics

Experiment attributes:

GEO Accession: GSM4663610

Links:

NCBI link: NCBI Entrez (gds)

Runs: 1 run, 47.7M spots, 9.5G bases, 5Gb

Run	# of Spots	# of Bases	Size	Published
SRR12183595	47,712,643	9.5G	5Gb	2021-01-15

ID:: 11319771

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