GSM4658439: ER-RAS Induced + shXPO7_3 D5shX3T; Homo sapiens; RNA-Seq - SRA

SRX8678448: GSM4658439: ER-RAS Induced + shXPO7_3 D5shX3T; Homo sapiens; RNA-Seq
2 ILLUMINA (Illumina HiSeq 2500) runs: 44M spots, 2.2G bases, 736.6Mb downloads

Submitted by: NCBI (GEO)

Study: Analysis of senescence transcriptome upon knockdown of XPO7 [RNA-seq]

PRJNA644552 • SRP270635 • All experiments • All runs

show Abstracthide Abstract

We depleted XPO7 by shRNA in IMR90 ER:RAS cells. 5 days after RAS induction by treatment with 4OHT we collected RNA. XPO7 depletion alleviates senescence induction. Overall design: 4 conditions in triplicate. IMR90 ER:RAS cells, either: non-induced control cells (non-senescent), 4OHT induced cells (Senescent, induced), IMR90 ER:RAS cells, 4OHT-induced and with XPO7 depletion (by expressing shXPO7_3), IMR90 ER:RAS cells, 4OHT-induced and with XPO7 depletion (by expressing shXPO7_5)

Sample: ER-RAS Induced + shXPO7_3 D5shX3T

SAMN15463294 • SRS6957527 • All experiments • All runs

Organism: Homo sapiens

Library:

Instrument: Illumina HiSeq 2500

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: SINGLE

Construction protocol: RNA was extracted using the Qiagen RNAeasy kit, following the instructions. Libraries were prepared according to Illumina's kit instructions (mRNA stranded library prep kit)

Experiment attributes:

GEO Accession: GSM4658439

Links:

NCBI link: NCBI Entrez (gds)

Runs: 2 runs, 44M spots, 2.2G bases, 736.6Mb

Run	# of Spots	# of Bases	Size	Published
SRR12162951	21,986,169	1.1G	368.1Mb	2021-02-12
SRR12162952	21,986,340	1.1G	368.5Mb	2021-02-12

ID:: 11293435

SRA

Sequence Read Archive

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