GSM4658434: ER-RAS Non-induced D5minusA; Homo sapiens; RNA-Seq - SRA

SRX8678443: GSM4658434: ER-RAS Non-induced D5minusA; Homo sapiens; RNA-Seq
2 ILLUMINA (Illumina HiSeq 2500) runs: 46.7M spots, 2.3G bases, 784.7Mb downloads

Submitted by: NCBI (GEO)

Study: Analysis of senescence transcriptome upon knockdown of XPO7 [RNA-seq]

PRJNA644552 • SRP270635 • All experiments • All runs

show Abstracthide Abstract

We depleted XPO7 by shRNA in IMR90 ER:RAS cells. 5 days after RAS induction by treatment with 4OHT we collected RNA. XPO7 depletion alleviates senescence induction. Overall design: 4 conditions in triplicate. IMR90 ER:RAS cells, either: non-induced control cells (non-senescent), 4OHT induced cells (Senescent, induced), IMR90 ER:RAS cells, 4OHT-induced and with XPO7 depletion (by expressing shXPO7_3), IMR90 ER:RAS cells, 4OHT-induced and with XPO7 depletion (by expressing shXPO7_5)

Sample: ER-RAS Non-induced D5minusA

SAMN15463299 • SRS6957522 • All experiments • All runs

Organism: Homo sapiens

Library:

Instrument: Illumina HiSeq 2500

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: SINGLE

Construction protocol: RNA was extracted using the Qiagen RNAeasy kit, following the instructions. Libraries were prepared according to Illumina's kit instructions (mRNA stranded library prep kit)

Experiment attributes:

GEO Accession: GSM4658434

Links:

NCBI link: NCBI Entrez (gds)

Runs: 2 runs, 46.7M spots, 2.3G bases, 784.7Mb

Run	# of Spots	# of Bases	Size	Published
SRR12162941	23,350,274	1.2G	392.1Mb	2021-02-12
SRR12162942	23,350,894	1.2G	392.6Mb	2021-02-12

ID:: 11293430

SRA

Sequence Read Archive

Result Filters

Send to:

Supplemental Content

Related information

Search details

Recent activity