Sequencing of therapeutic transposon insertions from TT1 mouse liver - SRA

SRX8562677: Sequencing of therapeutic transposon insertions from TT1 mouse liver
1 ILLUMINA (Illumina MiSeq) run: 5M spots, 1.3G bases, 729.7Mb downloads

Design: An alternative Tn-seq method in which specific PCR amplicons were enriched using streptavidin-coated paramagnetic beads.

Submitted by: Biological Research Centre

Study: Mouse transposon-based gene therapy

PRJNA605684 • SRP267602 • All experiments • All runs

show Abstracthide Abstract

NGS identification of vector integration sites from organs of TT1 mice underwent transposon-based gene therapy.Tyrosinemia Type I (TT1) is a fatal inherited metabolic disorder. We treated the mouse model of TT1 with transposon-based gene therapy and sequenced the genomic positions of gene delivery events from different organs.

Sample:

SAMN15235914 • SRS6855436 • All experiments • All runs

Organism: Mus musculus

Library:

Name: PB10_liver

Instrument: Illumina MiSeq

Strategy: Tn-Seq

Source: GENOMIC

Selection: PCR

Layout: PAIRED

Runs: 1 run, 5M spots, 1.3G bases, 729.7Mb

Run	# of Spots	# of Bases	Size	Published
SRR12031310	5,008,469	1.3G	729.7Mb	2023-03-21

ID:: 11129143

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