GSM4560793: F12O1_Liver_RNA-Seq; Xenopus tropicalis; RNA-Seq - SRA

SRX8367761: GSM4560793: F12O1_Liver_RNA-Seq; Xenopus tropicalis; RNA-Seq
1 ILLUMINA (Illumina Genome Analyzer IIx) run: 38.8M spots, 2.8G bases, 1,007Mb downloads

Submitted by: NCBI (GEO)

Study: Using RNAseq for identifying hepatic genes involved in multi- (F1 generation) and transgenerational (F2 generation) responses to chronic benzo[a]pyrene exposure (F0 generation) in Xenopus tropicalis

PRJNA634073 • SRP262433 • All experiments • All runs

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We investigated the multi- and transgenerational impact of BaP on Xenopus females following F0 generation exposure from the tadpole to the mature adult stage at a concentration (50 ng.L-1). 5 females from F1 and F2 generations by parentale xposure conditions (control vs BaP) were used for studying liver transcriptome by RNAseq. RNA-seq libraries were sequenced on an illumina GAIIx sequencer as 75bp single reads Overall design: mRNAseq from liver of mature F1 and F2 females.

Sample: F12O1_Liver_RNA-Seq

SAMN14979719 • SRS6683422 • All experiments • All runs

Organism: Xenopus tropicalis

Library:

Instrument: Illumina Genome Analyzer IIx

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: SINGLE

Construction protocol: For each biological replicate, the RNAqueous®-4PCR Kit (Ambion, Austin USA) was used to extract the total RNA from 15 mg of liver according to manufacturer instructions RNA-seq libraries were prepared using the TruSeq Stranded mRNA sample Prep kit (Illumina, San Diego, USA) and sequenced on an Illumina GAIIx sequencer as 75 bp reads

Experiment attributes:

GEO Accession: GSM4560793

Links:

NCBI link: NCBI Entrez (gds)

Runs: 1 run, 38.8M spots, 2.8G bases, 1,007Mb

Run	# of Spots	# of Bases	Size	Published
SRR11816742	38,769,048	2.8G	1,007Mb	2021-01-02

ID:: 10883051

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