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SRX8167855: GSM4494958: E12.0PG_ATACseq_rep3; Mus musculus; ATAC-seq
1 ILLUMINA (NextSeq 500) run: 29.3M spots, 2.2G bases, 874.6Mb downloads

Submitted by: NCBI (GEO)
Study: Organ of Corti size is governed by Yap/Tead-mediated progenitor self-renewal
show Abstracthide Abstract
We report on gene expression, chromatin accessibility, active histone marks distribution, and Tead DNA-binding in proliferating and postmitotic organ of Corti progenitor cells. Overall design: Examination of gene expression (RNA-seq), chromatin accessibility (ATAC), histone modifications (CUT&RUN), and Tead-binding (CUT&RUN) in 2 cell types.
Sample: E12.0PG_ATACseq_rep3
SAMN14682312 • SRS6529087 • All experiments • All runs
Organism: Mus musculus
Library:
Instrument: NextSeq 500
Strategy: ATAC-seq
Source: GENOMIC
Selection: other
Layout: PAIRED
Construction protocol: Progenitors (Sox2-GFP+) were purified from E12 cochlea by FACS sorting. Nuclei were extracted and chromatin were digested by tn5 transposase at 37°C for 20 minutes. DNA fragments were extracted using MiniElute columns (Qiagen), and then amplified by PCR. Libraries were amplified by PCR with indexed primers.
Experiment attributes:
GEO Accession: GSM4494958
Links:
Runs: 1 run, 29.3M spots, 2.2G bases, 874.6Mb
Run# of Spots# of BasesSizePublished
SRR1160125329,301,1802.2G874.6Mb2020-04-24

ID:
10645088

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