GSM4475103: Male_Saline_3_24h; Mus musculus; RNA-Seq - SRA

SRX8111440: GSM4475103: Male_Saline_3_24h; Mus musculus; RNA-Seq
2 ILLUMINA (Illumina NovaSeq 6000) runs: 52M spots, 5.3G bases, 1.5Gb downloads

Submitted by: NCBI (GEO)

Study: Neuroinflammatory astrocyte subtypes in the mouse brain [bulk RNA-seq]

PRJNA625202 • SRP256314 • All experiments • All runs

show Abstracthide Abstract

Bulk-seq of FACS purified astrcoytes from saline- and LPS-injected Aldh1l1-GFP mice across time and sex Overall design: Total RNA was purified from astrocytes from P30-35 Aldh1l1-GFP mice, FACS-puified and RNA collected for bulk-seq and sequenced on a NOVAseq 6000

Sample: Male_Saline_3_24h

SAMN14595052 • SRS6476514 • All experiments • All runs

Organism: Mus musculus

Library:

Instrument: Illumina NovaSeq 6000

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: PAIRED

Construction protocol: Astrocyte were FACS-purified and RNA extracted using the Agilent Absolutely Total RNA Purification Kit cDNA was generated using the SMART-seq HT kit and libraries were generated using the Nextera XT kit (Takara Bio).

Experiment attributes:

GEO Accession: GSM4475103

Links:

NCBI link: NCBI Entrez (gds)

Runs: 2 runs, 52M spots, 5.3G bases, 1.5Gb

Run	# of Spots	# of Bases	Size	Published
SRR11541236	25,858,677	2.6G	788.2Mb	2021-03-01
SRR11541237	26,099,281	2.7G	796Mb	2021-03-01

ID:: 10561306

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