GSM4405596: 2K_1; Homo sapiens; RNA-Seq - SRA

SRX7891866: GSM4405596: 2K_1; Homo sapiens; RNA-Seq
1 ILLUMINA (Illumina HiSeq 3000) run: 47.8M spots, 7.3G bases, 2.6Gb downloads

Submitted by: NCBI (GEO)

Study: KRAB domain of ZFP568 disrupts TRIM28-mediated abnormal interactions in cancer cells

PRJNA611924 • SRP252344 • All experiments • All runs

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We observed changes in protein levels of EZH2, TRIM24, p53, and a substantial number of KRAB-ZF proteins in MCF7 cells upon introducing a peptide fragment containing the KRAB domain. Overall design: mRNA profiles of MCF7 cells expressing Krab domain, knockdown of TRIM28, and Vector alone, sh scrambled control were generated using deep sequencing, in duplicate

Sample: 2K_1

SAMN14352210 • SRS6301002 • All experiments • All runs

Organism: Homo sapiens

Library:

Instrument: Illumina HiSeq 3000

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: PAIRED

Construction protocol: Qiagen mRNA kit Illumina TruSeq Stranded total RNA kit according to the manufacturer's protocol. The libraries were sequenced using 2x76 bases paired end protocol on Illumina HiSeq 3000 instrument. Two biological replicates per condition were sequenced, generating 45-58 million pairs of reads per sample. Each pair of reads represents a cDNA fragment from the library.

Experiment attributes:

GEO Accession: GSM4405596

Links:

NCBI link: NCBI Entrez (gds)

Runs: 1 run, 47.8M spots, 7.3G bases, 2.6Gb

Run	# of Spots	# of Bases	Size	Published
SRR11286157	47,798,544	7.3G	2.6Gb	2020-04-29

ID:: 10323220

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Sequence Read Archive

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