GSM4274267: SR_6h_1_1; Drosophila melanogaster; RNA-Seq - SRA

SRX7569542: GSM4274267: SR_6h_1_1; Drosophila melanogaster; RNA-Seq
1 BGISEQ (BGISEQ-500) run: 28.4M spots, 1.4G bases, 801.5Mb downloads

Submitted by: NCBI (GEO)

Study: Spatiotemporal female reproductive tract gene expression in Drosophila melanogaster

PRJNA601501 • SRP242106 • All experiments • All runs

show Abstracthide Abstract

In order to better understand the female reproductive tract (FRT) we conducted a systematic, comprehensive investigation of the FRT in a tssue-specific manner at three time points relative to mating. By characterizing the transcriptional relationships among discrete FRT tissues across time we advance the understanding of the molecular genetics of FRT functions. Overall design: RNAseq of five FRT tissues (bursa, oviduct, seminal receeptacle, spermatheca, and parovaria) as well as the FRT-associated fat body in unmated females as well as females 6hrs and 24hrs post-mating

Sample: SR_6h_1_1

SAMN13870076 • SRS6005571 • All experiments • All runs

Organism: Drosophila melanogaster

Library:

Instrument: BGISEQ-500

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: SINGLE

Construction protocol: Females were etherized (unmated, 6hrs, or 24hrs post-mating) and the female reproductive tract (FRT) removed in a drop of 1x PBS. The FRT was then microdissected into 6 discrete tissues. Tissues were rinsed in PBS and transferred directly to Trizol. Pooled tissue collections were homogenized with a pellet pestle and RNA was isolated using a phenol-chloroform extraction with phase-lock gels (ThermoFisher) and an overnight precipitation in isoproponal and 3M sodium acetate with 1.5ul glycogen (LifeTechnologies) at -18 C to maximize RNA recovery. The precipitation was followed by DNAse I (LifeTechnologies) treatment for 30 min at 37 C and a second RNA isolation. RNA was re-suspended in RNase-free H2O RNA libraries were prepared for sequencing using standard protocols by Beijing Genomics Institute (BGI)

Experiment attributes:

GEO Accession: GSM4274267

Links:

NCBI link: NCBI Entrez (gds)

Runs: 1 run, 28.4M spots, 1.4G bases, 801.5Mb

Run	# of Spots	# of Bases	Size	Published
SRR10901383	28,373,102	1.4G	801.5Mb	2020-12-30

ID:: 9888271

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