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SRX7290772: GSM4210666: NEBNext B 1; synthetic construct; miRNA-Seq
1 ILLUMINA (Illumina HiSeq 2500) run: 19.2M spots, 1.5G bases, 671Mb downloads

Submitted by: NCBI (GEO)
Study: An extension to: Systematic assessment of commercially available low-input miRNA library preparation kits
show Abstracthide Abstract
We compared the performance of two commercial kits (NEBNext and NEXTflex) for miRNA detection sensitivity, reliability, titration response and ability to detect differentially expressed miRNAs at different amounts of synthetic miRNAs. We observed differences in detection sensitivity between the kits, but none were able to detect the full repertoire of expected miRNAs. The reliability within the replicates of all kits was good, while larger differences were observed between the kits, although none could accurately quantify the majority of miRNAs. In a previous publication (https://doi.org/10.1080/15476286.2019.1667741; GEO accession number GSE133719) we accessed the performance of six additional miRNA library preparation kits. Overall design: Systematic comparison of miRNA profiles assessed in low-input synthetic miRNA samples and samples from pools of blood derived CD8 T cells of rheumatoid arthritis (RA) patients or healthy controls (HC) by six commercially available library preparation kits. In a previous publication (https://doi.org/10.1080/15476286.2019.1667741; GEO accession number GSE133719) we accessed the performance of six additional miRNA library preparation kits.
Sample: NEBNext B 1
SAMN13512561 • SRS5783960 • All experiments • All runs
Library:
Instrument: Illumina HiSeq 2500
Strategy: miRNA-Seq
Source: TRANSCRIPTOMIC
Selection: size fractionation
Layout: SINGLE
Construction protocol: Synthetic miRNA samples: no RNA extraction needed, human samples: RNA/DNA/Protein Purification Kit (Norgen Biotek Corp) NEBNext, NEXTflex
Experiment attributes:
GEO Accession: GSM4210666
Links:
Runs: 1 run, 19.2M spots, 1.5G bases, 671Mb
Run# of Spots# of BasesSizePublished
SRR1061150019,174,0351.5G671Mb2020-06-01

ID:
9567584

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