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SRX7241815: GSM4196671: 10M-Exp-Spleen-B-RNA; Mus musculus; RNA-Seq
1 ILLUMINA (Illumina HiSeq 4000) run: 52.9M spots, 15.9G bases, 6.1Gb downloads

Submitted by: NCBI (GEO)
Study: H1 linker histones regulate the balance of repressive and active chromatin domains via localized genomic compaction [RNA-seq]
show Abstracthide Abstract
H1 linker histones are the most abundant chromatin binding proteins. Their association with chromatin determines the spacing between nucleosomes and enables arrays of nucleosomes to fold into more compact chromatin structures. Mammals express multiple H1 proteins and are able to compensate for the loss of one or even two members by increasing synthesis of other members to maintain a constant H1 to nucleosome stoichiometry. To study the role of H1 in mammalian development, we generated a conditional triple H1 knockout (H1cTKO) mouse strain that enables depletion of H1 in specific cell types. Here, we report on the effects of depleting H1 in adult hematopoietic cells. Deletion of the genes encoding three widely expressed H1 subtypes (H1c, H1d, and H1e) has particularly profound effects on B- and T- lymphocyte development. H1 depletion leads to de-repression of T-cell activation genes, and a shift in T-cells towards effector functions, a process that mimics normal T-cell activation. Comparison of chromatin structure in normal and H1-depleted CD8+ T-cells revealed that H1 binding produces localized chromatin compaction within spatially defined chromatin domains containing above average levels of H1. Reduction of H1 stoichiometry in these regions leads to decreases in H3K27 methylation and increases in H3K36 methylation. In vitro, H1 promotes PRC2-mediated H3K27 methylation and inhibits NSD2-mediated H3K36 methylation. Mechanistically, H1 mediates these opposite effects by promoting physical compaction of the chromatin substrate. These findings identify H1 as a critical regulator of the epigenetic landscape in mammalian cells. Overall design: 5x10^5 B, and CD4+ and CD8+ T cells were sorted to Trizol by FACS. RNA seq libraries were prepared from polyA-enriched RNA by Genewiz.
Sample: 10M-Exp-Spleen-B-RNA
SAMN13429259 • SRS5741300 • All experiments • All runs
Organism: Mus musculus
Library:
Instrument: Illumina HiSeq 4000
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: PAIRED
Construction protocol: 5x10E5 B, and CD4+ and CD8+ T cells were sorted to Trizol by FACS. paired-end RNA seq libraries were prepared from polyA-enriched RNA by GeneWiz (https://www.genewiz.com/)
Experiment attributes:
GEO Accession: GSM4196671
Links:
Runs: 1 run, 52.9M spots, 15.9G bases, 6.1Gb
Run# of Spots# of BasesSizePublished
SRR1056011852,902,84015.9G6.1Gb2020-09-09

ID:
9508405

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