GSM4138320: Gyman-10m-2; Gymnopus androsaceus; RNA-Seq - SRA

SRX7051490: GSM4138320: Gyman-10m-2; Gymnopus androsaceus; RNA-Seq
2 ILLUMINA (Illumina HiSeq 2500) runs: 78.7M spots, 19.7G bases, 8.7Gb downloads

Submitted by: NCBI (GEO)

Study: Gymnopus androsaceus growth on pine needles

PRJNA579340 • SRP226835 • All experiments • All runs

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Illumina HiSeq2500 technology was used to generate mRNA profiles from Gymnopus androsaceus grown on pine needles for 2, 5 and 10 months. Paired-end reads of 125 bp were generated and aligned to Gymnopus androsaceus reference transcripts using CLC Genomics Workbench 9. Overall design: mRNA profiles from Gymnopus androsaceus grown on pine needles for 2, 5 and 10 months were generated by Illumina HiSeq2500 sequencing (2x125bp). Three biological replicates from 2, 5 and 10 months were sequenced.

Sample: Gyman-10m-2

SAMN13108453 • SRS5568350 • All experiments • All runs

Organism: Gymnopus androsaceus

Library:

Instrument: Illumina HiSeq 2500

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: PAIRED

Construction protocol: Total RNA was extracted from 800 mg of tissue per sample using the RNA PowerSoil Total RNA Isolation Kit (Mobio now Qiagen). Quantification and integrity check were conducted using an Experion Automated Electrophoresis Station (Bio-Rad, Hercules, CA, USA). cDNA libraries were prepared for sequencing using standard Illumina protocols by GeT (Génopole Toulouse Midi-Pyrénées, Auzeville, France)

Experiment attributes:

GEO Accession: GSM4138320

Links:

NCBI link: NCBI Entrez (gds)

Runs: 2 runs, 78.7M spots, 19.7G bases, 8.7Gb

Run	# of Spots	# of Bases	Size	Published
SRR10341456	38,820,948	9.7G	4.3Gb	2019-12-01
SRR10341457	39,873,027	10G	4.5Gb	2019-12-01

ID:: 9270530

SRA

Sequence Read Archive

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