GSM4114129: miRNA SS2; Gallus gallus; ncRNA-Seq - SRA

SRX6965770: GSM4114129: miRNA SS2; Gallus gallus; ncRNA-Seq
1 ILLUMINA (Illumina HiSeq 2500) run: 15.6M spots, 779.8M bases, 396.7Mb downloads

Submitted by: NCBI (GEO)

Study: Genome-wide studies of microRNAs in Marek's tumorigenesis in Chicken (miRNA-Seq)

PRJNA576489 • SRP224812 • All experiments • All runs

show Abstracthide Abstract

Marek's disease (MD), induced by Marek's disease virus (MDV), is a lymphotropic neoplastic disease and causes huge economic losses to the poultry industry. Non-coding RNAs play important regulatory roles in disease pathogenesis. To investigate host miRNA expression profile, RNA sequencing was performed in tumorous spleens (TS), spleens from the survivors (SS) without any lesion after MDV infection, and noninfected chicken spleens (NS). Overall design: we investigated the microRNA (miRNA) profiles in the Marker's tumorigenesis disease in chicken through sequencing 14 samples for different stages of Marek's disease and analyzing the RNA-seq data using existing software

Sample: miRNA SS2

SAMN12995059 • SRS5491566 • All experiments • All runs

Organism: Gallus gallus

Library:

Instrument: Illumina HiSeq 2500

Strategy: ncRNA-Seq

Source: TRANSCRIPTOMIC

Selection: size fractionation

Layout: SINGLE

Construction protocol: The total RNA was extracted from spleen tissues by Trizol (Invitrogen, CA) according to the manufacturer¡¯s instruction. A total of 3 ¦Ìg total RNA per sample was used as input material for the small RNA library. Sequencing libraries were generated using NEBNext? Multiplex Small RNA Library Prep Set for Illumina? (NEB, USA.) following the manufacturer¡¯s recommendations and index codes were added to attribute sequences to each sample.

Experiment attributes:

GEO Accession: GSM4114129

Links:

NCBI link: NCBI Entrez (gds)

Runs: 1 run, 15.6M spots, 779.8M bases, 396.7Mb

Run	# of Spots	# of Bases	Size	Published
SRR10247304	15,596,089	779.8M	396.7Mb	2020-01-21

ID:: 9163556

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