GSM4086432: kit Input; Mus musculus; ChIP-Seq - SRA

SRX6874369: GSM4086432: kit Input; Mus musculus; ChIP-Seq
1 ILLUMINA (HiSeq X Ten) run: 27.3M spots, 8.2G bases, 3.2Gb downloads

Submitted by: NCBI (GEO)

Study: Ultra-low-input native ChIP-seq data of four germ-cell types during mouse spermatogenesis

PRJNA566429 • SRP222567 • All experiments • All runs

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We applied ultra-low-input native ChIP-seq and profiled genome-wide H3K9me3 distribution in four germ cell types during spermatogenesis. Overall design: Whole-genome H3K9me3 modifications of mouse spermatogenesis was deeply sequenced using Illumina HiSeq X10 system.

Sample: kit Input

SAMN12788961 • SRS5410275 • All experiments • All runs

Organism: Mus musculus

Library:

Instrument: HiSeq X Ten

Strategy: ChIP-Seq

Source: GENOMIC

Selection: ChIP

Layout: PAIRED

Construction protocol: All sorted cells and H3K9me3 antibody incubated by magnetic beads were immunoprecipitated and DNA was finally isolated. The amount of DNA used for the ChIP: 6pg. The sequence libraries were generated using the KAPA Hyper Prep Kit for the Illumina platform (kk8504), following the manufacturer's instructions. Paired-end 150-bp sequencing was performed on Illumina HiSeq X10.

Experiment attributes:

GEO Accession: GSM4086432

Links:

NCBI link: NCBI Entrez (gds)

Runs: 1 run, 27.3M spots, 8.2G bases, 3.2Gb

Run	# of Spots	# of Bases	Size	Published
SRR10148915	27,308,461	8.2G	3.2Gb	2020-07-18

ID:: 9045960

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