GSM3945664: PD9-2; Homo sapiens; RNA-Seq - SRA

SRX6454837: GSM3945664: PD9-2; Homo sapiens; RNA-Seq
1 ILLUMINA (Illumina HiSeq 3000) run: 33.5M spots, 6.8G bases, 2.5Gb downloads

Submitted by: NCBI (GEO)

Study: RNA-seq in putamen of Parkinson's disease patients

PRJNA555099 • SRP215209 • All experiments • All runs

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We performed RNA-seq on total RNA from tissue samples from putamen of the brain from Parkinson's disease patients. Overall design: Total RNA was extracted from tissue samples from ten Parkinson's disease patients. rRNA was depleted before sequencing. Samples were sequenced in duplicates, creating two paired-end experiments per sample. contributor: Harvard Brain Tissue Resource Center

Sample: PD9-2

SAMN12306275 • SRS5122113 • All experiments • All runs

Organism: Homo sapiens

Library:

Instrument: Illumina HiSeq 3000

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: PAIRED

Construction protocol: Whole brains were slabbed and frozen at -80 °C. RNA was extracted using AllPrep DNA/RNA/miRNA Universal Kit (Qiagen; #80224). 300 ng RNA per sample was used as inpt for library preparation using TruSeq Stranded Total RNA Sample Preparation Kit with Ribo-Zero Gold rRNA Removal Kit (Illumina; #RS-122-2303).

Experiment attributes:

GEO Accession: GSM3945664

Links:

NCBI link: NCBI Entrez (gds)

Runs: 1 run, 33.5M spots, 6.8G bases, 2.5Gb

Run	# of Spots	# of Bases	Size	Published
SRR9696540	33,471,972	6.8G	2.5Gb	2020-02-20

ID:: 8551355

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