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SRX6423019: GSM3935791: UPF1_isoform_2_RIP-Seq_2; Homo sapiens; RIP-Seq
1 ILLUMINA (Illumina HiSeq 3000) run: 73.5M spots, 11G bases, 4.1Gb downloads

Submitted by: NCBI (GEO)
Study: UPF1 regulatory loop variant RNA-Seq and RIP-Seq
show Abstracthide Abstract
HEK-293 Trex cells containing stable tetracycline-inducible transgenes driving overexpression of CLIP-tagged UPF1 isoforms 1 and 2 or GFP were used for total RNA-seq. In addition, CLIP-UPF1 isoforms were affinity purified, and bound RNA was subjected to high-throughput sequencing. Overall design: Paired-end high-throughput RNA sequencing of Total RNA from cells expressing CLIP-tagged UPF1 isoform 1, UPF1 isoform 2, or GFP and RNA associated with affinity-purified CLIP UPF1 isoforms 1 and 2.
Sample: UPF1_isoform_2_RIP-Seq_2
SAMN12239785 • SRS5079810 • All experiments • All runs
Organism: Homo sapiens
Library:
Instrument: Illumina HiSeq 3000
Strategy: RIP-Seq
Source: TRANSCRIPTOMIC
Selection: other
Layout: PAIRED
Construction protocol: Qiagen RNeasy Mini RNA isolation kit One μg of total RNA was used per sample for library preparation. Ribosomal RNA was depleted from total RNA with the Ribo-Zero rRNA Removal Kit (Epicentre), and purified RNA was then converted to cDNA with the TruSeq RNA Library Preparation Kit (Illumina).
Experiment attributes:
GEO Accession: GSM3935791
Links:
Runs: 1 run, 73.5M spots, 11G bases, 4.1Gb
Run# of Spots# of BasesSizePublished
SRR966214473,492,77511G4.1Gb2020-05-28

ID:
8507584

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